High-throughput micro-fermentation for directed evolution and strain selection in industrial biotechnology

用于工业生物技术中定向进化和菌株选择的高通量微发酵

• 批准号: BB/R000069/1
• 负责人: Daniela Delneri
• 金额: $ 27.32万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR000069%2F1
• 关键词: throughput; micro; fermentation; directed; evolution

Scientific advances in industrial biotechnology (IB) and bio-based chemicals production have the potential to transform the global industrial landscape revolutionising sustainable manufacturing processes across all industrial sectors, including healthcare, sustainable energy, green chemistry, pharmaceuticals, novel materials and bioremediation. In fact, many of the challenges faced by industry and society (including energy demand and efficiency, replacement of fossil fuels and dealing with pollution, resource efficiency, food security, climate change and energy shortages) can now be tackled by bio-based production technologies. To exploit the potential of IB a thorough understanding of the performance of production strains and scale-up processes is critical.The high-throughput bench top micro-fermentation system (Robolector-XL) provides a solution to a major challenge area of process/clone validation prior to extensive scale up studies. The RoboLector-XL (and other related systems) are now in extensive use by several industrial concerns and this has radically altered their approach to cell line development. This capability would provide a critical enabling technology to several areas of biotechnological research of focus within both the Manchester Institute of Biotechnology (MIB) and the wider University of Manchester (UoM) research environment, and will help drive the research towards exploitation and commercialisation.

工业生物技术（IB）和生物基化学品生产的科学进步有可能改变全球工业格局，彻底改变所有工业部门的可持续制造工艺，包括医疗保健、可持续能源、绿色化学、制药、新材料和生物修复。事实上，工业和社会面临的许多挑战（包括能源需求和效率、化石燃料的替代和处理污染、资源效率、粮食安全、气候变化和能源短缺）现在都可以通过生物生产技术来解决。为了充分利用IB的潜力，对生产菌株的性能和放大工艺的全面了解至关重要。高通量台式微发酵系统（Robolector-XL）为广泛的放大研究之前的工艺/克隆验证的主要挑战领域提供了解决方案。RoboLector-XL（和其他相关系统）现在被几个工业企业广泛使用，这从根本上改变了他们的细胞系开发方法。这种能力将为曼彻斯特生物技术研究所（MIB）和更广泛的曼彻斯特大学（UoM）研究环境中的几个生物技术研究领域提供关键的使能技术，并将有助于推动研究开发和商业化。

项目成果

Transcriptional Profile of the Industrial Hybrid Saccharomyces pastorianus Reveals Temperature-Dependent Allele Expression Bias and Preferential Orthologous Protein Assemblies.

• DOI: 10.1093/molbev/msab282
• 发表时间: 2021-12-09
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: Timouma S;Balarezo-Cisneros LN;Pinto J;De La Cerda R;Bond U;Schwartz JM;Delneri D
• 通讯作者: Delneri D

Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and in trans effects on the protein regulatory network.

• DOI: 10.1371/journal.pgen.1008761
• 发表时间: 2021-01
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Balarezo-Cisneros LN;Parker S;Fraczek MG;Timouma S;Wang P;O'Keefe RT;Millar CB;Delneri D
• 通讯作者: Delneri D

Restoring fertility in yeast hybrids: Breeding and quantitative genetics of beneficial traits.

• DOI: 10.1073/pnas.2101242118
• 发表时间: 2021-09-21
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Naseeb S;Visinoni F;Hu Y;Hinks Roberts AJ;Maslowska A;Walsh T;Smart KA;Louis EJ;Delneri D
• 通讯作者: Delneri D