权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Vertical-stage confocal microscopy for live imaging of growing plants

用于生长植物实时成像的立式共焦显微镜

基本信息

批准号：
BB/R000859/1
负责人：
Stefan Kepinski
金额：
$ 20.67万
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR000859%2F1
关键词：
Vertical stage confocal microscopy live

项目摘要

As all gardeners know, plant roots grow vertically downwards, and they do this by responding to gravity. Understanding how this happens, how roots develop and respond to their environment is essential to improving nutrient and water uptake in plants, which will ultimately help us improve agricultural productivity to feed the world. Plant growth and development are complex processes that are regulated by several proteins and other molecules within cells. Understanding where in the cells these proteins normally reside, and how they move within cells either during normal developmental processes or in response to environmental stimuli is an important first step in understanding the molecular pathways that regulate plant growth responses, and microscopy is a common tool used to study this. However, most microscopes are only equipped with a horizontal stage, which means that plants cannot be imaged in their natural vertical orientiation. As plants respond to gravity, placing them in a horizontal orientation perturbs their normal behaviour, making it difficult to draw valid conclusions about the normal plant response to gravity. The new horizontally-mounted confocal microscope will allow us, for the first time, to use powerful confocal microscopy to image vertical plant roots in their natural orientation at high resolution (with fine detail). This cutting edge vertical microscope will enable us to image proteins and other molecules in their native orientation within cells, as well as allow the dynamic live imaging of movement of these molecules in response to different environmental signals and during growth and development.

正如所有的园丁都知道的那样，植物的根垂直向下生长，它们通过响应重力来做到这一点。了解这是如何发生的，根系如何发育并对环境做出反应，对于改善植物的营养和水分吸收至关重要，这将最终帮助我们提高农业生产力，养活世界。植物的生长和发育是一个复杂的过程，受细胞内多种蛋白质和其他分子的调控。了解这些蛋白质通常在细胞中的位置，以及它们在正常发育过程中或响应环境刺激时如何在细胞内移动，是了解调节植物生长反应的分子途径的重要第一步，显微镜是用于研究的常用工具。然而，大多数显微镜只配备了一个水平的阶段，这意味着植物不能在其自然的垂直方向成像。由于植物对重力有反应，将它们水平放置会扰乱它们的正常行为，因此很难就植物对重力的正常反应得出有效的结论。新的水平安装共聚焦显微镜将使我们能够首次使用强大的共聚焦显微镜以高分辨率（精细细节）在自然方向上对垂直植物根部进行成像。这种尖端的垂直显微镜将使我们能够在细胞内对蛋白质和其他分子的天然方向进行成像，并允许对这些分子响应不同环境信号以及在生长和发育过程中的运动进行动态实时成像。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Antigravitropic PIN polarization maintains non-vertical growth in lateral roots.

DOI：
10.1038/s41477-023-01478-x
发表时间：
2023-09
期刊：
NATURE PLANTS
影响因子：
18
作者：
Roychoudhry, Suruchi;Sageman-Furnas, Katelyn;Wolverton, Chris;Grones, Peter;Tan, Shutang;Molnar, Gergely;De Angelis, Martina;Goodman, Heather L.;Capstaff, Nicola;Lloyd, James P. B.;Mullen, Jack;Hangarter, Roger;Friml, Jiri;Kepinski, Stefan
通讯作者：
Kepinski, Stefan

The Analysis of Gravitropic Setpoint Angle Control in Plants.

植物向重力设定点角度控制的分析。

DOI：
10.1007/978-1-0716-1677-2_10
发表时间：
2022
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
0
作者：
Roychoudhry S
通讯作者：
Roychoudhry S

Rice actin binding protein RMD controls crown root angle in response to external phosphate.

水稻肌动蛋白结合蛋白 RMD 响应外部磷酸盐控制冠根角度

DOI：
10.1038/s41467-018-04710-x
发表时间：
2018-06-11
期刊：
Nature communications
影响因子：
16.6
作者：
Huang G;Liang W;Sturrock CJ;Pandey BK;Giri J;Mairhofer S;Wang D;Muller L;Tan H;York LM;Yang J;Song Y;Kim YJ;Qiao Y;Xu J;Kepinski S;Bennett MJ;Zhang D
通讯作者：
Zhang D

Internalization of rabies virus glycoprotein differs between pathogenic and attenuated virus strains.

DOI：
10.1099/jgv.0.001935
发表时间：
2023-12
期刊：
JOURNAL OF GENERAL VIROLOGY
影响因子：
3.8
作者：
Almasoud, Ibrahim;Charlton, Frank W.;Finke, Stefan;Barr, John N.;Mankouri, Jamel
通讯作者：
Mankouri, Jamel

Stress-induced F-Box protein-coding gene OsFBX257 modulates drought stress adaptations and ABA responses in rice.

胁迫诱导的 F-Box 蛋白编码基因 OsFBX257 调节水稻的干旱胁迫适应和 ABA 反应。