DIACYLGLYCEROL & C-KINASE AT INTERACELLULAR MEDIATORS

胰岛素调节机体代谢功能的机制 目标组织尚不清楚。特别是，细胞内的 介导葡萄糖胰岛素效应的信号系统 尚未定义传输。最近的许多发现表明， 二酰甘油-C-激酶信号系统可能发挥作用 胰岛素诱导的血糖升高过程中的重要作用 运输和其他代谢功能。概括地说， 研究结果：(A)胰岛素刺激磷脂代谢， 二酰甘油生成和激活C-激酶；(B)C-激酶 激活(通过各种方式)可激发胰岛素样效应。 葡萄糖转运、氨基酸转运与丙酮酸 脱氢酶激活；以及(C)葡萄糖转运体是 被C-激酶磷酸化。目前的研究提案将 进一步研究双甘油-C-激酶的假说 信号系统作为胰岛素的细胞内介体 对葡萄糖运输和其他代谢过程的影响。在……里面 特别是，我们将确定(A)胰岛素是如何增加的 二酰甘油含量；(B)胰岛素如何增加C-激酶活性； (C)靶组织，其中胰岛素增加二酰甘油和C- 和(D)胰岛素的更重要后果-- 诱导二酰甘油和C-激酶的升高。

The mechanisms whereby insulin regulates metabolic functions in target tissues are unknown. In particular, the intracellular signaling system that mediates the insulin effect of glucose transport has not been defined. Many recent findings suggest that the diacylglycerol-C-kinase signaling system could play an important role during insulin-induced increases in glucose transport and other metabolic functions. To summarize these findings: (a) insulin stimulates phospholipid metabolism, diacylglycerol generation and activation of C-kinase; (b) C-kinase activation (by various means) provokes insulin-like effects on glucose transport, amino acid transport and pyruvate dehydrogenase activation; and (c) the glucose transporter is phosphorylated by C-kinase. The present research proposal will study further the hypothesis that the diacylglycerol-C-kinase signaling system serves as an intracellular mediator for insulin effects on glucose transport and other metabolic processes. In particular, we will determine (a) how insulin increases diacylglycerol content; (b) how insulin increases C-kinase activity; (c) target tissues wherein insulin increases diacylglycerol and C- kinase; and (d) the more important consequences of insulin- induced increases in diacylglycerol and C-kinase.