A versatile proton transfer reaction-mass spectrometry platform for online monitoring of VOCs.

用于在线监测 VOC 的多功能质子转移反应质谱平台。

基本信息

批准号：
BB/R013497/1
负责人：
Nigel Scrutton
金额：
$ 57.73万
依托单位：
University of Manchester
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR013497%2F1
关键词：
versatile proton transfer reaction mass

项目摘要

Proton-transfer-reaction mass spectrometry (PTR-MS) is a specialized branch of mass spectrometry that is used for online monitoring of volatile organic compounds (VOCs). Commercially available PTR-MS instruments have a response time of about 100 ms and reach a detection limit in the single digit pptv region. It uses gas phase hydronium ions as ion source reagents. This makes PTR-MS spectrometers ideally suited for rapid and sensitive analysis of VOCs in a wide range of bioscience research programmes enabled by the online, simultaneous real-time detection, monitoring and quantification of VOCs. PTR-MS uses the 'soft' ionisation technique of proton transfer from protonated water (H3O+), which is produced as an ion beam by an ion source. During this interaction a proton transfers from the hydronium to the trace gas molecule of interest that leads to a protonated and hence ionised molecule. This reaction is energetically possible for all VOCs with a proton affinity higher than that of water and is thus amenable to alkanes, alkenes, alkynes, aromatics and other organic molecules; PTR-MS therefore provides good coverage of the volatilome. This coverage can be further enhanced with the use of different reagent gases; this is available via fast switching and includes O2+, NO+, Kr+ and Xe+. Furthermore, combining the unique PTR ionisation with Time-of-Flight MS permits for high-sensitivity and high-resolution detection. PTR-MS thus offers major advantages over current analysis methods and it meets many of the demands of contemporary bioscience programmes such as rapid continuous monitoring.The ability to monitor chemical diversity of the volatilome in situ and in real-time provided by the requested PTR-MS platform will provide major new insights towards our understanding of the underpinning mechanisms of biological systems. This aligns with BBSRC strategic priorities by underpinning research programmes that tackle major challenges in synthetic biology, bioenergy, food nutrition and health, integrative microbiome research and strategic approaches to industrial biotechnology (IB).

质子转化反应质谱（PTR-MS）是质谱的专业分支，用于在线监测挥发性有机化合物（VOC）。市售的PTR-MS仪器的响应时间约为100 ms，并且在单位数字PPTV区域中达到检测极限。它使用气相氢离子作为离子源试剂。这使得PTR-MS光谱仪非常适合在线，同时实时检测，监视和量化VOC的广泛生物科学研究计划中对VOC的快速和敏感分析。 PTR-MS使用质子水（H3O+）的质子转移的“软”电离技术，该技术由离子源作为离子束生产。在这种相互作用期间，质子从氢转移到感兴趣的痕量气体分子，从而导致质子化并因此导致电离分子。对于所有具有高于水的质子亲和力的VOC，这种反应在能量上都是可能的，因此可以适合烷烃，烷烃，炔烃，芳香族和其他有机分子。因此，PTR-MS可以很好地覆盖挥发组。使用不同的试剂气体可以进一步增强这种覆盖范围。通过快速开关可用，包括O2+，NO+，KR+和XE+。此外，将唯一的PTR离子化与飞行时间MS允许相结合，以进行高敏性和高分辨率检测。因此，PTR-MS比当前的分析方法具有重要的优势，并且它符合当代生物科学计划的许多需求，例如快速连续监测。监测挥发性型的化学多样性以及所需的PTR-MS平台提供的实时提供的能力，将为我们对我们对生物学系统的基础机制的理解提供主要的新见解。这与BBSRC战略优先事项相吻合，该计划的基础是应对合成生物学，生物能源，食品营养和健康，综合微生物组研究以及工业生物技术（IB）的战略方法的研究计划。