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A versatile proton transfer reaction-mass spectrometry platform for online monitoring of VOCs.

用于在线监测 VOC 的多功能质子转移反应质谱平台。

基本信息

批准号：
BB/R013497/1
负责人：
Nigel Scrutton
金额：
$ 57.73万
依托单位：
University of Manchester
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR013497%2F1
关键词：
versatile proton transfer reaction mass

项目摘要

Proton-transfer-reaction mass spectrometry (PTR-MS) is a specialized branch of mass spectrometry that is used for online monitoring of volatile organic compounds (VOCs). Commercially available PTR-MS instruments have a response time of about 100 ms and reach a detection limit in the single digit pptv region. It uses gas phase hydronium ions as ion source reagents. This makes PTR-MS spectrometers ideally suited for rapid and sensitive analysis of VOCs in a wide range of bioscience research programmes enabled by the online, simultaneous real-time detection, monitoring and quantification of VOCs. PTR-MS uses the 'soft' ionisation technique of proton transfer from protonated water (H3O+), which is produced as an ion beam by an ion source. During this interaction a proton transfers from the hydronium to the trace gas molecule of interest that leads to a protonated and hence ionised molecule. This reaction is energetically possible for all VOCs with a proton affinity higher than that of water and is thus amenable to alkanes, alkenes, alkynes, aromatics and other organic molecules; PTR-MS therefore provides good coverage of the volatilome. This coverage can be further enhanced with the use of different reagent gases; this is available via fast switching and includes O2+, NO+, Kr+ and Xe+. Furthermore, combining the unique PTR ionisation with Time-of-Flight MS permits for high-sensitivity and high-resolution detection. PTR-MS thus offers major advantages over current analysis methods and it meets many of the demands of contemporary bioscience programmes such as rapid continuous monitoring.The ability to monitor chemical diversity of the volatilome in situ and in real-time provided by the requested PTR-MS platform will provide major new insights towards our understanding of the underpinning mechanisms of biological systems. This aligns with BBSRC strategic priorities by underpinning research programmes that tackle major challenges in synthetic biology, bioenergy, food nutrition and health, integrative microbiome research and strategic approaches to industrial biotechnology (IB).

质子转移反应质谱（PTR-MS）是质谱的一个专门的分支，用于在线监测挥发性有机化合物（VOCs）。市售的PTR-MS仪器具有约100 ms的响应时间，并且在个位数pptv区域中达到检测限。它使用气相水合氢离子作为离子源试剂。这使得PTR-MS光谱仪非常适合在广泛的生物科学研究项目中对VOC进行快速灵敏的分析，这些项目通过在线，同时实时检测，监测和定量VOC来实现。PTR-MS使用从质子化水（H3 O+）转移质子的“软”电离技术，质子化水由离子源产生为离子束。在这种相互作用期间，质子从水合氢转移到感兴趣的痕量气体分子，从而导致质子化并因此电离分子。该反应对于质子亲和力高于水的所有VOC都是可能的，因此适合烷烃、烯烃、炔烃、芳香族化合物和其他有机分子;因此PTR-MS提供了对挥发物组的良好覆盖。通过使用不同的试剂气体可以进一步增强这种覆盖范围;这可以通过快速切换实现，包括O2+、NO+、Kr+和NH3+。此外，将独特的PTR电离与飞行时间MS相结合，可以实现高灵敏度和高分辨率的检测。因此，PTR-MS提供了当前分析方法的主要优势，它满足了当代生物科学计划的许多需求，例如快速连续监测。所要求的PTR-MS平台提供的原位和实时监测挥发物组化学多样性的能力将为我们理解生物系统的基础机制提供重要的新见解。这与BBSRC的战略优先事项相一致，支持应对合成生物学，生物能源，食品营养和健康，综合微生物组研究和工业生物技术战略方法（IB）的重大挑战的研究计划。