Towards a Bio-based Manufacturing Platform for High Strength Aramid (Aromatic Polyamide) Synthetic Fibres Using Synthetic Biology

利用合成生物学构建高强度芳纶（芳香族聚酰胺）合成纤维的生物基制造平台

• 批准号: EP/N025504/1
• 负责人: Nigel Scrutton
• 金额: $ 51.08万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FN025504%2F1
• 关键词: Towards; Bio; based; Manufacturing; Platform

There is a pressing need to unite and extend capabilities to take biomaterials synthesis to a new level, by developing strategies that enable rapid assembly of biological polymers and their subsequent (targeted) chemical elaboration to generate a diverse range of bio-derived materials. The game changer is the ability to re-write DNA to encode modules / polymers of interest and/or to design assembly production lines that enable rapid exploration of combinatorial monomer/polymer space with massive expansion in biomaterials diversity.Creation of microscopic living foundries to produce and secrete materials (and their building blocks) with properties that can be genetically encoded is embedded in the new science of synthetic biology. Automation is critical to explore and facilitate the modular construction and combinatorial assembly of synthetic DNA. Re-writing DNA draws inspiration from refactoring, a process used to improve computer software. Synthetic biologists use refactoring to re-build natural systems, from the ground up, to provide engineered surrogates that are easier to understand. Better understanding facilitates predictable engineering, a key objective in the emerging field of synthetic biomaterials which is founded on the underlying principles of the new biology termed synthetic biology.Synthetic biology can drive next generation synthetic biomaterials discovery, production and manufacture, and there is a pressing need to do so. The new biology harnesses synthetic and systems biology, and leverages engineering expertise and the integration of biotechnology, evolutionary biology, chemical engineering, molecular biology and genetic engineering. The step-change here is rapid development of bio-based production methods for scale-up / scale-out using a variety of production hosts (e.g. mammalian and fermentation approaches; natural hosts) that enable rapid isolation and chemical / biological elaboration of new materials. Because of the combinatorial approach there is a need for automated high throughput assembly of these production pipelines, and the the possible outcomes are almost limitless. In this proposal we embrace these new technologies to give access to the microscopic living factories that are capable of synthesizing the building blocks for established and new aramid-based polymers that have widespread civilian and military applications. We aim to develop a scaled versatile production platform, which accommodates rapid ultra high throughput screening of polymer building blocks prior to more intensive downstream recovery of selected building blocks from expression hosts. The emerging science and technology will facilitate sustainable manufacture of established and new aramid polymers that will translate also to similar production platforms for other established or novel biologically derived materials. Our approach should also lead to the discovery of new aramid polymer properties that will create opportunities for their use in existing and new military or civilian applications.

迫切需要联合和扩展能力，通过制定策略，使生物聚合物的快速组装和随后的（有针对性的）化学加工能够产生各种各样的生物衍生材料，将生物材料合成提升到一个新的水平。改变游戏规则的是重新编写DNA以编码感兴趣的模块/聚合物和/或设计组装生产线的能力，这些生产线能够快速探索组合单体/聚合物空间，并大规模扩展生物材料的多样性。创造微小的活铸造厂来生产和分泌具有遗传编码特性的材料（及其构建模块）是合成生物学这门新科学的一部分。自动化对于探索和促进合成DNA的模块化构建和组合组装至关重要。重写DNA从重构中获得灵感，重构是一种用于改进计算机软件的过程。合成生物学家使用重构来从头开始重建自然系统，以提供更容易理解的工程替代品。更好的理解有助于可预测的工程，这是合成生物材料新兴领域的一个关键目标，它建立在被称为合成生物学的新生物学的基本原理之上。合成生物学可以推动下一代合成生物材料的发现、生产和制造，并且迫切需要这样做。新的生物学利用合成生物学和系统生物学，并利用工程专业知识和生物技术，进化生物学，化学工程，分子生物学和基因工程的整合。这里的逐步变化是生物基生产方法的快速发展，利用各种生产宿主（例如哺乳动物和发酵方法；自然宿主）进行规模化/规模化生产，从而实现新材料的快速分离和化学/生物细化。由于组合方法，需要对这些生产管道进行自动化高通量装配，并且可能的结果几乎是无限的。在这个方案中，我们采用了这些新技术，以使微观生活工厂能够合成具有广泛民用和军事应用的现有和新型芳纶基聚合物的基石。我们的目标是开发一个规模化的多功能生产平台，该平台可以在从表达宿主中更密集地下游回收选定的构建块之前，对聚合物构建块进行快速超高通量筛选。新兴的科学和技术将促进现有的和新的芳纶聚合物的可持续生产，这也将转化为其他现有的或新的生物衍生材料的类似生产平台。我们的方法还将导致发现新的芳纶聚合物特性，这将为其在现有和新的军事或民用应用中创造机会。

项目成果

Building a minimal and generalizable model of transcription factor-based biosensors: Showcasing flavonoids.

• DOI: 10.1002/bit.26726
• 发表时间: 2018-09
• 期刊: Biotechnology and bioengineering
• 影响因子: 3.8
• 作者: Trabelsi H;Koch M;Faulon JL
• 通讯作者: Faulon JL

Graphene-aramid nanocomposite fibres via superacid co-processing.

• DOI: 10.1039/c9cc04548a
• 发表时间: 2019-09
• 期刊: Chemical communications
• 影响因子: 4.9
• 作者: A. Roberts;Paul P. Kelly;Jennifer Bain;J. Morrison;I. Wimpenny;Mike Barrow;Robert T. Woodward;M. Gresil;C. Blanford;Sam Hay;J. Blaker;S. Yeates;N. Scrutton

Molecular structures enumeration and virtual screening in the chemical space with RetroPath2.0.

• DOI: 10.1186/s13321-017-0252-9
• 发表时间: 2017-12-19
• 期刊: Journal of cheminformatics
• 影响因子: 8.6
• 作者: Koch M;Duigou T;Carbonell P;Faulon JL
• 通讯作者: Faulon JL