Towards a Bio-based Manufacturing Platform for High Strength Aramid (Aromatic Polyamide) Synthetic Fibres Using Synthetic Biology

利用合成生物学构建高强度芳纶（芳香族聚酰胺）合成纤维的生物基制造平台

• 批准号: EP/N025504/1
• 负责人: Nigel Scrutton
• 金额: $ 51.08万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FN025504%2F1
• 关键词: Towards; Bio; based; Manufacturing; Platform

There is a pressing need to unite and extend capabilities to take biomaterials synthesis to a new level, by developing strategies that enable rapid assembly of biological polymers and their subsequent (targeted) chemical elaboration to generate a diverse range of bio-derived materials. The game changer is the ability to re-write DNA to encode modules / polymers of interest and/or to design assembly production lines that enable rapid exploration of combinatorial monomer/polymer space with massive expansion in biomaterials diversity.Creation of microscopic living foundries to produce and secrete materials (and their building blocks) with properties that can be genetically encoded is embedded in the new science of synthetic biology. Automation is critical to explore and facilitate the modular construction and combinatorial assembly of synthetic DNA. Re-writing DNA draws inspiration from refactoring, a process used to improve computer software. Synthetic biologists use refactoring to re-build natural systems, from the ground up, to provide engineered surrogates that are easier to understand. Better understanding facilitates predictable engineering, a key objective in the emerging field of synthetic biomaterials which is founded on the underlying principles of the new biology termed synthetic biology.Synthetic biology can drive next generation synthetic biomaterials discovery, production and manufacture, and there is a pressing need to do so. The new biology harnesses synthetic and systems biology, and leverages engineering expertise and the integration of biotechnology, evolutionary biology, chemical engineering, molecular biology and genetic engineering. The step-change here is rapid development of bio-based production methods for scale-up / scale-out using a variety of production hosts (e.g. mammalian and fermentation approaches; natural hosts) that enable rapid isolation and chemical / biological elaboration of new materials. Because of the combinatorial approach there is a need for automated high throughput assembly of these production pipelines, and the the possible outcomes are almost limitless. In this proposal we embrace these new technologies to give access to the microscopic living factories that are capable of synthesizing the building blocks for established and new aramid-based polymers that have widespread civilian and military applications. We aim to develop a scaled versatile production platform, which accommodates rapid ultra high throughput screening of polymer building blocks prior to more intensive downstream recovery of selected building blocks from expression hosts. The emerging science and technology will facilitate sustainable manufacture of established and new aramid polymers that will translate also to similar production platforms for other established or novel biologically derived materials. Our approach should also lead to the discovery of new aramid polymer properties that will create opportunities for their use in existing and new military or civilian applications.

通过制定能够快速组装生物学聚合物及其随后的（有针对性的）化学阐述，以产生各种生物衍生的材料，因此需要将生物材料合成的能力团结起来，以将生物材料合成至新水平。改变游戏规则的能力是重新编码感兴趣的模块/聚合物和/或设计组装生产线的模块/聚合物，从而可以快速探索与生物材料多样性中大量扩展的组合单体/聚合物空间的综合空间。生物学。自动化对于探索和促进合成DNA的模块化结构和组合组合至关重要。重新编写的DNA从重构中汲取灵感，这是用于改善计算机软件的过程。合成生物学家使用重构从头开始重建天然系统，以提供更容易理解的工程替代物。更好的理解促进了可预测的工程，这是基于称为合成生物学的新生物学的基本原理的合成生物材料新兴领域的关键目标。ShyntheticBiology可以推动下一代合成生物材料的发现，生产和制造，并且需要迫切需要这样做。新生物学利用合成和系统生物学，并利用工程专业知识以及生物技术，进化生物学，化学工程，分子生物学和基因工程的整合。这里的逐步变化是使用各种生产宿主（例如哺乳动物和发酵方法；自然寄主）快速开发基于生物的生产方法，以扩大 /扩展，从而可以快速分离和化学 /生物学阐述新材料。由于采用组合方法，需要这些生产管道的自动高吞吐量组件，并且可能的结果几乎是无限的。在此提案中，我们采用这些新技术，以访问能够为建立的基于广泛的平民和军事应用的建立和新的基于Aramid的聚合物合成基础的微观生活工厂。我们旨在开发一个规模的多功能生产平台，该平台可容纳聚合物构建块的快速超高吞吐量筛选，然后再从表达宿主那里进行更密集的下游恢复所选构件。新兴的科学技术将促进既定和新的芳香聚合物的可持续生产，这些聚合物还将转化为其他已建立或新颖的生物学衍生材料的类似生产平台。我们的方法还应导致发现新的芳香聚合物特性，这将为它们在现有和新的军事或平民应用中的使用创造机会。

项目成果

Building a minimal and generalizable model of transcription factor-based biosensors: Showcasing flavonoids.

• DOI: 10.1002/bit.26726
• 发表时间: 2018-09
• 期刊: Biotechnology and bioengineering
• 影响因子: 3.8
• 作者: Trabelsi H;Koch M;Faulon JL
• 通讯作者: Faulon JL

Graphene-aramid nanocomposite fibres via superacid co-processing.

• DOI: 10.1039/c9cc04548a
• 发表时间: 2019-09
• 期刊: Chemical communications
• 影响因子: 4.9
• 作者: A. Roberts;Paul P. Kelly;Jennifer Bain;J. Morrison;I. Wimpenny;Mike Barrow;Robert T. Woodward;M. Gresil;C. Blanford;Sam Hay;J. Blaker;S. Yeates;N. Scrutton

Molecular structures enumeration and virtual screening in the chemical space with RetroPath2.0.

• DOI: 10.1186/s13321-017-0252-9
• 发表时间: 2017-12-19
• 期刊: Journal of cheminformatics
• 影响因子: 8.6
• 作者: Koch M;Duigou T;Carbonell P;Faulon JL
• 通讯作者: Faulon JL