ION TRANSPORT IN COLLECTING CELL CULTURES
ION TRANSPORT IN COLLECTING CELL CULTURES
批准号:
2140623
负责人:
ELSA N. BELLO-REUSS
金额:
$17.78万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-08-01 至 1994-11-30
关键词:
aldosterone basolateral membrane cell type dopamine electrophysiology epithelium hormone regulation /control mechanism ion transport kidney cell laboratory rabbit membrane permeability membrane potentials microelectrodes mixed tissue /cell culture neurotransmitters norepinephrine potassium renal tubule acidosis
中文摘要
我们的目的是了解离子的输运性质及其机制
英文摘要
We aim to understand the ion transport properties and the mechanisms of its
regulation in the different cell types of the cortical collecting duct
(CCD). Specific aims are: 1. Continue characterizing the ion-transport
mechanisms expressed in CCD cells in culture. Mixed CCD cultures will be
used to identify mechanisms of ion transport, and the effects of ADH,
aldosterone, and isoproterenol on the different transporters. 2. To study
the effects of intra and extracellular pH on the cell biology and transport
functions of CCD monolayers. 2.1. To assess the modifications in the
proportion of the different cell types and the morphological changes in
response to alterations in acid-base status of the cultures. 2.2. To
establish the pH dependence of the transporters located in the apical and
basolateral membranes of the different cells. We will examine the
conductive and nonconductive ion-transport pathways of the three cell types
under basal conditions and their responses to acute and chronic changes in
pH of basolateral and/or apical solutions. 3. To characterize,
structurally and functionally, homogeneous monolayers of principal cells
and intercalated cells. 3.1. The ultrastructure and function of principal-
cell monolayers will be studied: We will characterize at the cell-membrane
level the baseline ion transport mechanisms and their regulation by
hormones (ADH, aldosterone, isoproterenol). 3.2. Homogeneous cultures of
intercalated cells, initially identified as either alpha or beta cells,
will be studied with and without hormonal stimulation. Special
consideration will be given to the possibility of transformation of a cell
type to another. Monolayers of CCD cells will allow us to gather basic
information on ion transport mechanisms at the cellular and single-membrane
levels. They should also be useful tools for biochemical and molecular
studies. Homogenous cultures will permit quantitative studies without the
confounding influences of neighboring cells of different type. These
studies could help our understanding of the pathogenesis of renal tubule
ion-transport defects, such as renal tubular acidosis, concentration
defects and K+ secretion abnormalities.
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Characterization of acid-base transport mechanisms in the kidney cell line RCCT-28A.
肾细胞系 RCCT-28A 中酸碱转运机制的表征。
DOI:
10.1038/ki.1993.29
发表时间:
1993
期刊:
Kidney international
影响因子:
19.6
作者:
[Bello-Reuss,E]
通讯作者:
Bello-Reuss,E
Xenobiotic transport differences in mouse mesangial cell clones expressing mdr1 and mdr3.
表达 mdr1 和 mdr3 的小鼠系膜细胞克隆中的异生素转运差异。
DOI:
10.1152/ajpcell.1996.270.3.c910
发表时间:
1996
期刊:
The American journal of physiology
影响因子:
--
作者:
[Ernest,S, Bello-Reuss,E]
通讯作者:
Bello-Reuss,E
Electrophysiological studies on primary cultures of proximal tubule cells.
近曲小管细胞原代培养物的电生理学研究。
DOI:
10.1152/ajprenal.1986.251.3.f490
发表时间:
1986
期刊:
The American journal of physiology
影响因子:
--
作者:
[Bello-Reuss,E, Weber,MR]
通讯作者:
Weber,MR
Characterization of peanut-lectin (+) cells derived from the RCCT-28A cell line.
源自 RCCT-28A 细胞系的花生凝集素 ( ) 细胞的表征。
DOI:
10.1038/ki.1993.30
发表时间:
1993
期刊:
Kidney international
影响因子:
19.6
作者:
[Bello-Reuss,E]
通讯作者:
Bello-Reuss,E
Electrophysiological studies of primary cultures of rabbit distal tubule cells.
兔远端肾小管细胞原代培养物的电生理学研究。
DOI:
10.1152/ajprenal.1987.252.5.f899
发表时间:
1987
期刊:
The American journal of physiology
影响因子:
--
作者:
[Bello-Reuss,E, Weber,MR]
通讯作者:
Weber,MR
共 6 条
FUNCTION OF P-GLYCOPROTEIN IN KIDNEY CELLS
-
批准号:2838143
-
项目类别:
-
资助金额:$24.86万
-
财政年份:1996
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
FUNCTION OF P-GLYCOPROTEIN IN KIDNEY CELLS
-
批准号:2016822
-
项目类别:
-
资助金额:$24.19万
-
财政年份:1996
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
FUNCTION OF P-GLYCOPROTEIN IN KIDNEY CELLS
-
批准号:2608460
-
项目类别:
-
资助金额:$24.25万
-
财政年份:1996
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT & ITS REGULATION IN PROXIMAL RENAL TUBULES
-
批准号:3238162
-
项目类别:
-
资助金额:$9.53万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT IN COLLECTING CELL CULTURES
-
批准号:3238160
-
项目类别:
-
资助金额:$15.12万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT IN COLLECTING CELL CULTURES
-
批准号:3238163
-
项目类别:
-
资助金额:$15.53万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT IN COLLECTING CELL CULTURES
-
批准号:3238165
-
项目类别:
-
资助金额:$16.89万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT IN COLLECTING CELL CULTURES
-
批准号:3238164
-
项目类别:
-
资助金额:$15.84万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT & ITS REGULATION IN PROXIMAL RENAL TUBULES
-
批准号:3238166
-
项目类别:
-
资助金额:$3.74万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT & ITS REGULATION IN PROXIMAL RENAL TUBULES
-
批准号:3238161
-
项目类别:
-
资助金额:$9.38万
-
财政年份:1986
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT & ITS REGULATION IN PROXIMAL RENAL TUBULES
-
批准号:3231751
-
项目类别:
-
资助金额:$5.38万
-
财政年份:1983
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
ION TRANSPORT & ITS REGULATION IN PROXIMAL RENAL TUBULES
-
批准号:3152793
-
项目类别:
-
资助金额:$8.95万
-
财政年份:1983
-
负责人:ELSA N. BELLO-REUSS
-
依托单位:
海外基金