Towards on-column monitoring of protein purification by ATR-FTIR and Raman Spectroscopy

通过 ATR-FTIR 和拉曼光谱对蛋白质纯化进行柱上监测

• 批准号: BB/R019533/1
• 负责人: Bernadette Byrne
• 金额: $ 25.39万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR019533%2F1
• 关键词: Towards; column; monitoring; protein; purification

Biopharmaceuticals, proteins used as drugs, are an emerging area in the treatment of a range of diseases including rheumatoid arthritis and cancer. While these therapeutics have been shown to be very effective, they are also extremely expensive (~£10,000s/treatment), due to the complex, large scale production processes required. The most expensive step is Protein A affinity capture. Previous research by our group has utilised a specialized technique called Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy to explore the causes of Protein A resin decay. The research outlined in this proposal aims to further characterise binding capacity decay using this approach and also develop the approach to make this a tool that will be useful to scientists in an industrial bioprocessing environment. Our direct monitoring approach will provide much greater control into Protein A purification and has the potential to extend resin life time, saving production costs.

生物制药是一种用作药物的蛋白质，是治疗包括类风湿性关节炎和癌症在内的一系列疾病的新兴领域。虽然这些疗法已被证明非常有效，但由于所需的复杂、大规模的生产工艺，它们也极其昂贵(~10000 GB/次)。最昂贵的步骤是蛋白A亲和捕获。我们团队之前的研究利用了一种名为衰减全反射傅里叶变换红外光谱(ATR-FTIR)的专门技术来探索蛋白A树脂腐烂的原因。这项提案中概述的研究旨在使用这种方法进一步表征结合能力的衰减，并开发一种方法，使其成为一种对工业生物处理环境中的科学家有用的工具。我们的直接监测方法将为蛋白A的纯化提供更好的控制，并有可能延长树脂的使用寿命，节省生产成本。

项目成果

Vibrational Spectroscopy in Protein Research

蛋白质研究中的振动光谱学

• 发表时间: 2020
• 作者: Byrne, B

Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy.

• DOI: 10.1039/d1an00985k
• 发表时间: 2021-08-09
• 期刊: The Analyst
• 作者: Beattie JW;Rowland-Jones RC;Farys M;Tran R;Kazarian SG;Byrne B
• 通讯作者: Byrne B

Application of Raman Spectroscopy to Dynamic Binding Capacity Analysis.

• DOI: 10.1177/00037028231210293
• 发表时间: 2023-12
• 期刊: Applied spectroscopy
• 影响因子: 3.5

Causes of Industrial Protein A Column Degradation, Explored Using Raman Spectroscopy.

• DOI: 10.1021/acs.analchem.2c03063
• 发表时间: 2022-11-15
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Beattie JW;Istrate A;Lu A;Marshall C;Rowland-Jones RC;Farys M;Kazarian SG;Byrne B
• 通讯作者: Byrne B

sj-docx-1-asp-10.1177_00037028231210293 - Supplemental material for Application of Raman Spectroscopy to Dynamic Binding Capacity Analysis

sj-docx-1-asp-10.1177_00037028231210293 - 拉曼光谱在动态结合能力分析中应用的补充材料

• DOI: 10.25384/sage.24487412
• 发表时间: 2023
• 作者: Beattie J