Reagentless Sensor Technologies For Continuous Monitoring of Heart Failure Biomarkers

用于连续监测心力衰竭生物标志物的无试剂传感器技术

• 批准号: 10636089
• 负责人: IGOR R EFIMOV
• 金额: $ 76.97万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-20 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10636089
• 关键词: Acute; Adhesions; Adhesives; Adopted; Adult; Affect; American; Anesthesia procedures; Animal Model; Benchmarking; Biochemical; Biological; Biological Assay; Biological Markers; Biological Monitoring; Blood; Buffers; Bulla; Cardiac health; Caregivers; Cells; Cellular Phone; Charge; Chronic; Chronic Care; Clinic; Clinic Visits; Clinical; Complex; Computer software; Data; Data Collection; Deposition; Dermis; Detection; Deterioration; Development; Devices; Diagnosis; Disease; Disease Management; Economics; Electrocardiogram; Electrodes; Electronics; Engineering; Environmental Monitoring; Enzyme-Linked Immunosorbent Assay; Enzymes; Exhibits; FDA approved; Filtration; Gold; Head; Health Personnel; Health Status; Healthcare; Heart Rate; Heart failure; Hospitalization; Hospitals; Hydrogels; Hydrophobicity; Implant; In Situ; In Vitro; Individual; Injections; Intercellular Fluid; Interleukin-1 beta; Interleukin-6; Kinetics; Measurement; Membrane; Metals; Methods; Modeling; Molecular; Monitor; Natural regeneration; Needles; Nucleic Acids; Operative Surgical Procedures; Oxygen; Patients; Performance; Peripheral; Persons; Pharmaceutical Preparations; Phosphate Buffer; Photoplethysmography; Platinum; Polymers; Population; Positioning Attribute; Proteins; Protocols documentation; Pulmonary artery structure; Pump; Rattus; Reagent; Recurrence; Reporter; Reporting; Risk; Risk Assessment; Running; Saline; Sampling; Shapes; Site; Skin; Specificity; Stress; Suction; Surgical Models; System; TNF gene; Technology; Testing; Textiles; Thinness; Time; Titrations; United States; Validation; Vertebral column; Wistar Rats; Work; aorta constriction; automated analysis; biomarker identification; biomarker panel; biomaterial compatibility; computer code; design; detection platform; electrical measurement; experience; flexibility; heart function; hospital readmission; hospitalization rates; implantation; implanted sensor; in vivo; in vivo monitoring; instrumentation; monitoring device; monolayer; nano; novel strategies; porous hydrogel; preservation; pressure; prevent; pro-brain natriuretic peptide (1-76); protein biomarkers; prototype; remote monitoring; sensor; sensor technology; smartphone based device; transmission process; wearable device; wearable monitor; wearable platform; wearable sensor technology; wireless; wireless transmission

Project Summary: Heart failure affects approximately twenty-six million people globally and represents a major economic and societal burden. This includes more than six million adults in the United States alone, a number that is predicted to rise by 46% over the next 15 years. Heart failure is a progressive condition characterized by recurrent exacerbations. Prior work indicates that biomarker monitoring can aid in the assessment of risk and provide meaningful data in management of the disease. But in order to allow this to be done on a continuous basis, self- regenerating sensors that can dynamically and continuously detect biomolecular species as a wearable system remain an unmet need. One major challenge to fully realize these systems is the development of platforms that allow for reagent-free analysis of complex biomolecules such as proteins, nucleic acids, and metabolites in real time. We recently developed the first reagentless sensor that can be adapted to any protein and demonstrated the ability to perform continuous monitoring in vivo using an implantable sensor array. In this project, we will adapt our technology for markers of heart failure to provide a new approach for remote monitoring of at-risk heart failure patients. The technology that enables this is a recently developed an enzyme-free and regent-free electrochemical reporter system that exhibits levels of sensitivity suitable for analysis of biological samples without the need for pre-processing. Electrochemical sensors are a powerful platform for environmental or biological monitoring based on inexpensive instrumentation and straightforward measurements of electrical currents. In the proposed project we will integrate these electrochemical sensors into biocompatible probes for in situ biochemical monitoring. We will test the sensors for sensitive and robust detection of a panel markers identified to be clinical indicators of cardiac health. Subsequently we will engineer these probes into a wearable platform capable of continuous monitoring of biomarkers present in the interstitial fluid. Requisite hardware and software for controlling the wearable monitoring device will be developed and tested in the project and automated analysis will be validated in an animal model of heart failure. The project deliverables will include first-in class biomarker monitoring systems with the potential to have a large impact on the American population by providing continual biomarker monitoring in healthcare for chronic conditions.

项目概要： 心力衰竭影响全球大约2600万人，并且代表了一个主要的经济和社会问题。 社会负担。这包括仅在美国就有600多万成年人， 将在未来15年内增长46%心力衰竭是一种进行性疾病，其特征是反复发作， 加重以前的工作表明，生物标志物监测可以帮助评估风险，并提供 对疾病管理有意义的数据。但为了使这一点能够在持续的基础上进行，自我- 再生传感器，可以动态和连续地检测生物分子物种作为可穿戴系统 仍然是一个未满足的需求。充分实现这些系统的一个主要挑战是开发平台， 允许在真实的中对复杂的生物分子如蛋白质、核酸和代谢物进行无试剂分析 时间 我们最近开发了第一个无试剂传感器，可以适应任何蛋白质，并证明了 使用可植入传感器阵列在体内进行连续监测的能力。在这个项目中，我们将适应 我们的心力衰竭标志物技术为高危心脏病的远程监测提供了一种新的方法 失败的病人这项技术是最近开发的一种无酶无试剂的 显示出适合于分析生物样品的灵敏度水平的电化学报告系统 而不需要预处理。电化学传感器是一个强大的平台，环境或 生物监测的基础上廉价的仪器和直接测量的电气 电流。 在拟议的项目中，我们将把这些电化学传感器集成到生物相容性探针中， 生化监测我们将测试传感器对已识别的面板标记物的灵敏和稳健检测 作为心脏健康的临床指标。随后，我们将把这些探针设计成一个可穿戴平台 能够连续监测组织液中存在的生物标志物。所需硬件和软件 用于控制可穿戴监测设备将开发和测试的项目和自动化分析 将在心力衰竭的动物模型中得到验证。该项目的可交付成果将包括一流的生物标志物 通过提供持续的监测系统，有可能对美国人口产生重大影响。 用于慢性病医疗保健的生物标志物监测。