IBD--ROLE OF ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES

建立溃疡性结肠炎诊断的重要性 在两个层面上。新近发病的急性特发性溃疡性结肠炎必须 与各种类似它的疾病不同。第二, 溃疡性结肠炎必须与克罗恩病相鉴别。这个 溃疡性结肠炎与克罗恩病携带者的鉴别 重要的预后和治疗意义。开发一种 方便可靠的血液检测，有助于溃烂的诊断 结肠炎和可能与疾病活动平行或预测和即将到来的病情 将是一个重要的进步。在其他免疫介导的情况下 疾病，血清自身抗体一直是主要的诊断用途，并 提供了对潜在疾病发病机制的见解。在某些情况下， 自身抗体已被用来揭示其分子结构/功能。 细胞内分子。自身抗体在分子水平和水平上的研究 细胞水平现在被视为一个重要的“桥梁” 和基础科学。我们最近发现了一组抗中性粒细胞 大多数溃疡性疾病患者的胞浆自身抗体 结肠炎。这一与UC相关的ANCA不同于 最初在韦格纳肉芽肿患者中报道，使用 描述了酶联免疫吸附试验。该项目的目标是评估临床 ANCA作为溃疡性结肠炎疾病标志物的应用 中性粒细胞胞浆抗原(S)对TO的识别和特性 ANCA是反应性的，并定义 组成ANCA的免疫球蛋白。除了促进 溃疡性结肠炎诊断标记物的研究进展 将提供对后续工作至关重要的信息 免疫生成与调控机制的研究进展 ANCA的。

The importance of establishing the diagnosis of ulcerative colitis arises at two levels. Acute idiopathic ulcerative colitis of recent onset must be distinguished from a variety of disorders that mimic it. Second, ulcerative colitis must be distinguished from Crohn's disease. The distinction between ulcerative colitis and Crohn's disease carries important prognostic and therapeutic implications. Development of a convenient and reliable blood test which could aid in diagnosing ulcerative colitis and might parallel disease activity or predict and impending flare would be an important advance. In other immunologically mediated disorders, serum autoantibodies have been of major diagnostic use and have provided insights into the underlying disease pathogenesis. In some cases, autoantibodies have been used to reveal the molecular structure/function of intracellular molecules. The study of autoantibodies at the molecular and cellular level is now viewed as an important "bridge between the clinical and basic sciences". We have recently found a subset of anti-neutrophil cytoplasmic autoantibodies in the majority of patients with ulcerative colitis. This UC-related ANCA is distinct from the ANCA which was originally reported in patients with Wegener's granulomatosus, using the ELISA assay described. The goal of this project is to assess the clinical utility of ANCA as a disease marker for ulcerative colitis, establish the identity and characteristics of the neutrophil cytoplasmic antigen(s) to which ANCA is reactive, and to define the characteristics of the immunoglobulins which comprise ANCA. In addition to facilitating the development of a diagnostic marker of ulcerative colitis, these studies will provide information which will be critical for subsequent investigations of the immunologic mechanisms of production and regulation of ANCA.