ROLE OF THE RPE IN DISK MEMBRANE RENEWAL

RPE 在椎间盘膜更新中的作用

• 批准号: 3256200
• 负责人: MICHAEL W KAPLAN
• 金额: $ 19.96万
• 依托单位: GOOD SAMARITAN HOSP & MED CTR(PRTLND,OR)
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-08-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3256200
• 关键词: Xenopus; cell death; cell membrane; cyclic GMP; eye regeneration; growth factor; histogenesis; human tissue; immunocytochemistry; intracellular transport; ischemia; membrane activity; membrane biogenesis; morphology; organ culture; protein transport; retina; retina detachment; retinal pigment epithelium; retinoids; rod cell; transducin; western blottings

The long term objective of this project is to understand the role that communication between retinal pigment epithelial (RPE) cells and photoreceptor cells plays in the normal renewal of photoreceptor disk membranes, and how renewal is affected by isolating the retina from the RPE. Several Important functions and metabolic processes of retinal photoreceptor cells depend upon the apposition of the retina with healthy RPE cells. Pronounced changes In the morphology and function of photoreceptor cells can be produced by Isolating the retina from the RPE or by dystrophies that affect RPE cells. The consequences of such changes in vivo may be irreversible, and the resulting loss of vision may be permanent. The etiology of changes produced in photoreceptors and RPE cells by retinal detachment from the RPE are not known. In an organ culture model, we find that isolating Xenopus retinas from the RPE results in aberrant morphogenesis of disk membranes. In contrast, disk assembly in co-cultured attached retinas is normal (as measured by several morphometric techniques). The specific aims of this project are Intended to answer several questions about the critical role that apposition of the retina with the RPE may play in the regulation of disk membrane assembly and morphogenesis: 1) Are processes of cell metabolism, gene expression and protein synthesis in photoreceptors affected? 2) What basic cell biological mechanisms are modified by isolating retinas from the RPE to cause abnormal disk assembly and morphogenesis? 3) Are effects of Isolating cultured retinas from the RPE reversible, either by re-establishing contact with the RPE or by restoring levels of potential trophic factors normally found in the subretinal space?

该项目的长期目标是了解 视网膜色素上皮（RPE）细胞和 感光细胞在感光盘的正常更新中发挥作用 膜，以及如何通过将视网膜与视网膜隔离来影响更新 视网膜色素上皮。 视网膜的几个重要功能和代谢过程 感光细胞依赖于视网膜与健康细胞的并置 视网膜色素上皮细胞。 形态和功能发生明显变化 感光细胞可以通过将视网膜与 RPE 分离来产生 或影响 RPE 细胞的营养不良。 这样的后果 体内的变化可能是不可逆的，由此导致的视力丧失可能 是永久的。 光感受器和 RPE 细胞产生变化的病因学 视网膜从 RPE 脱离尚不清楚。 在器官培养中 模型中，我们发现将爪蟾视网膜与 RPE 分离会导致 盘膜形态发生异常。 相比之下，磁盘组件在 共培养附着的视网膜是正常的（通过几个测量 形态测量技术）。 该项目的具体目标是 回答关于并置的关键作用的几个问题 视网膜和RPE可能参与视盘膜的调节 组装和形态发生：1）是细胞代谢、基因的过程 光感受器的表达和蛋白质合成受到影响吗？ 2) 什么 通过将视网膜从细胞中分离出来，可以改变基本的细胞生物学机制。 RPE导致椎间盘组装和形态发生异常？ 3）是否有效果 从可逆的 RPE 中分离培养的视网膜，可以通过 重新建立与 RPE 的联系或恢复电位水平 通常在视网膜下腔中发现的营养因子？