GeneORator: a novel and high-throughput method for the synthetic biology-based improvement of any enzyme

GeneORator：一种新颖的高通量方法，用于基于合成生物学的任何酶的改进

• 批准号: BB/S004955/1
• 负责人: Douglas Kell
• 金额: $ 25.73万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS004955%2F1
• 关键词: GeneORator; novel; throughput; method; synthetic

Enzymes have significant uses in biotechnology, for instance as components of 'biological' washing powders. The total enzyme market is large, some $8Bn per year.Natural enzymes are normally very poor. We can improve them by changing their sequences. However, this still scales as powers of their size: for 14 residues the number is greater than the lifetime in s of the known Universe (~100,000,000,000,000,000). We have a new method that allows one to do this additively (for 14 residues it is just 280 examples). This allows us to study the possible variants MUCH more effectively; with one enzyme we have made a variant that is 1210 times quicker than the starting enzyme.However, we now need to show that this was not a 'fluke' by demonstrating our methods on a series of other enzymes, and, as well as automating our methods using robots, this is what this project will do. This should get us to a state where we can attract sufficient investment to commercial our discoveries properly.

酶在生物技术中有重要的用途，例如作为“生物”洗衣粉的成分。酶的市场总量很大，每年约80亿美元。天然酶通常很差。我们可以通过改变它们的序列来改进它们。然而，这仍然是它们大小的幂：对于14个残基，其数量大于已知宇宙的寿命（~ 100，000，000，000，000）。我们有一个新的方法，允许一个这样做的加法（14个残基，它只是280个例子）。这使我们能够更有效地研究可能的变体;我们已经用一种酶制造了一种比起始酶快1210倍的变体。然而，我们现在需要通过在一系列其他酶上展示我们的方法来证明这不是“侥幸”，并且，以及使用机器人自动化我们的方法，这就是这个项目将要做的。这应该会让我们达到一个能够吸引足够投资的状态，以便将我们的发现适当地商业化。

项目成果

SynBiopython: an open-source software library for Synthetic Biology

• DOI: 10.1093/synbio/ysab001
• 发表时间: 2021-02-22
• 期刊: Synthetic Biology
• 影响因子: 3.2
• 作者: Yeoh JW;Swainston N;Vegh P;Zulkower V;Carbonell P;Holowko MB;Peddinti G;Poh CL
• 通讯作者: Poh CL

EnzymeML - a data exchange format for biocatalysis and enzymology

EnzymeML - 生物催化和酶学的数据交换格式

• DOI: 10.33774/chemrxiv-2021-bkxzw
• 发表时间: 2021
• 作者: Range J

The automated Galaxy-SynBioCAD pipeline for synthetic biology design and engineering.

• DOI: 10.1038/s41467-022-32661-x
• 发表时间: 2022-08-29
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Herisson, Joan;Duigou, Thomas;du Lac, Melchior;Bazi-Kabbaj, Kenza;Azad, Mahnaz Sabeti;Buldum, Gizem;Telle, Olivier;El Moubayed, Yorgo;Carbonell, Pablo;Swainston, Neil;Zulkower, Valentin;Kushwaha, Manish;Baldwin, Geoff S.;Faulon, Jean-Loup
• 通讯作者: Faulon, Jean-Loup

DNA Scanner: a web application for comparing DNA synthesis feasibility, price and turnaround time across vendors

DNA Scanner：用于比较不同供应商的 DNA 合成可行性、价格和周转时间的 Web 应用程序

• DOI: 10.1093/synbio/ysaa011
• 发表时间: 2020
• 期刊: Synthetic Biology
• 影响因子: 3.2
• 作者: Doçi G