Development, production and testing of novel glycoconjugate pig vaccines

新型复合糖猪疫苗的开发、生产和测试

• 批准号: BB/S004963/1
• 负责人: Brendan Wren
• 金额: $ 71.07万
• 依托单位: London School of Hygiene & Tropical Medicine
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS004963%2F1
• 关键词: Development; production; testing; novel; glycoconjugate

Healthily maintained livestock are essential for economic and societal prosperity. We propose to exploit ground-breaking technology to produce low cost glycoconjugate vaccines to protect against three major pig diseases (porcine pleuropneumonia, Glässer's disease and S. suis-induced meningitis), where current vaccines are unsatisfactory. We have developed technology to generate cloned glycoconjugates in E. coli cells that act as mini factories to produce an inexhaustible and renewal supply of pure vaccine. Due to production costs, conventional chemically synthesised glycoconjugate vaccines have not been used for animals, but have an excellent track record in humans, where over a billion doses have been used. Through the LSHTM business development office and industrial collaborators we will develop, produce and test new vaccines against the three major pig respiratory diseases. A new company, ArcVax, will be spun-out to further develop the technology and commercialise vaccine product.

健康饲养的牲畜对经济和社会繁荣至关重要。我们建议利用突破性的技术生产低成本的糖结合疫苗，以预防三种主要的猪疾病（猪胸膜肺炎、Glässer病和猪链球菌引起的脑膜炎），目前的疫苗不能令人满意。我们已经开发出在大肠杆菌细胞中产生克隆糖缀合物的技术，这些细胞就像微型工厂一样，生产出取之不尽、用之不竭的纯疫苗。由于生产成本的原因，传统的化学合成糖结合疫苗尚未用于动物，但在人类身上有着良好的记录，已使用了超过10亿剂。通过LSHTM业务发展办公室和行业合作伙伴，我们将开发、生产和测试针对三种主要猪呼吸道疾病的新疫苗。一家名为ArcVax的新公司将成立，以进一步开发该技术并将疫苗产品商业化。

项目成果

Additional file 1 of Engineering a suite of E. coli strains for enhanced expression of bacterial polysaccharides and glycoconjugate vaccines

工程化一套大肠杆菌菌株以增强细菌多糖和糖复合物疫苗的表达的附加文件 1

• DOI: 10.6084/m9.figshare.19634695
• 发表时间: 2022
• 作者: Kay E

Multivalent poultry vaccine development using Protein Glycan Coupling Technology.

• DOI: 10.1186/s12934-021-01682-4
• 发表时间: 2021-10-02
• 期刊: Microbial cell factories
• 影响因子: 6.4
• 作者: Mauri M;Sannasiddappa TH;Vohra P;Corona-Torres R;Smith AA;Chintoan-Uta C;Bremner A;Terra VS;Abouelhadid S;Stevens MP;Grant AJ;Cuccui J;Wren BW;Glycoengineering of Veterinary Vaccines Consortium
• 通讯作者: Glycoengineering of Veterinary Vaccines Consortium

Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction.

• DOI: 10.3389/fvets.2021.686088
• 发表时间: 2021
• 期刊: Frontiers in veterinary science
• 影响因子: 3.2
• 作者: Majorova D;Atkins E;Martineau H;Vokral I;Oosterhuis D;Olinga P;Wren B;Cuccui J;Werling D
• 通讯作者: Werling D

PglB function and glycosylation efficiency is temperature dependent when the pgl locus is integrated in the Escherichia coli chromosome.

• DOI: 10.1186/s12934-021-01728-7
• 发表时间: 2022-01-05
• 期刊: Microbial cell factories
• 影响因子: 6.4
• 作者: Terra VS;Mauri M;Sannasiddappa TH;Smith AA;Stevens MP;Grant AJ;Wren BW;Cuccui J;Glycoengineering of Veterinary Vaccines consortium (GoVV)
• 通讯作者: Glycoengineering of Veterinary Vaccines consortium (GoVV)

Ferric Citrate Regulator FecR Is Translocated across the Bacterial Inner Membrane via a Unique Twin-Arginine Transport-Dependent Mechanism.

柠檬酸铁调节剂 FecR 通过独特的双精氨酸运输依赖机制跨细菌内膜转运。

• DOI: 10.1128/jb.00541-19
• 发表时间: 2020
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Passmore IJ