权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

EFFECTS OF LIGHT ON THE RETINA

光对视网膜的影响

基本信息

批准号：
3256737
负责人：
HARRY G SPERLING
金额：
$ 3.07万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
起止时间：
1977-07-01 至 1987-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3256737
关键词：
color blindness color visions electron microscopy fresh water environment histochemistry /cytochemistry histology light adaptations operant conditionings psychophysics retinal adaptation visual photoreceptor

项目摘要

Repeated daily exposures of rhesus monkey eyes by an operant conditioning technique to spectral lights 2 to 3 log units less intense than the threshold intensity for gross burn damage produce loss of blue cone response after blue light exposure lasting longer than three years and of green cone response after green light exposure lasting between 18 and 45 days. Red light exposures within 10 percent of the gross burn threshold produce only 24 hour effects. Retinal sections of the eyes of the "blue-blinded" monkeys show swollen pigment epithelium and cone damage which ranges from swelling of mitochondria and axonal processes to total degeneration. In comparing the exposed with the unexposed eyes, there also appear to be cones completely missing in vertical 1 micron thick retinal sections in the parafoveal region. A new technique in our laboratory, using a histochemical marker in conjunction with spectral light exposure, has succeeded in labeling the complete color-receptor mosaic of the goldfish and is now underway successfully on the primate. It is proposed to continue this research with: (a) continued parametric studies of wavelength-intensity-duration relationships for producing the effect using goldfish and rhesus monkeys; (b) continued histological study using light and electron microscopy to attempt to identify the primary lesion in photic damage; (c) application of the histochemical technique to obtaining firm evidence on which retinal elements are involved as a function of wavelength and energy of exposure.

操作性条件反射对恒河猴眼的每日重复暴露对低于阈值强度的2到3个对数单位的光进行光谱处理的技术严重烧伤强度导致蓝色后蓝色锥体反应的损失持续三年以上的光照和绿色锥体反应绿灯照射持续18至45天。红灯曝光范围内 10%的严重烧伤阈值只会产生24小时的影响。蓝盲猴子眼睛的视网膜切片显示出肿胀的色素上皮和锥体损伤，包括线粒体和轴突肿胀完全退化的过程。在比较暴露的人和未暴露的人眼睛，垂直1微米处也似乎完全没有锥体黄斑旁区域有较厚的视网膜切片。我们实验室的一项新技术，将组织化学标记与用光谱光曝光，已经成功地标记了完整的金鱼的颜色感受器镶嵌，现在正在成功地灵长类。建议继续进行这项研究：(A)继续进行参数研究用于产生效果的波长-强度-持续时间关系金鱼和恒河猴；(B)继续用光和电子显微镜下试图识别原发病变中的光损伤； (C)应用组织化学技术获取关于哪些视网膜成分是作为波长和能量的函数参与的曝光。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sensitivity of the blue-sensitive cones across the central retina.

中央视网膜上蓝色敏感视锥细胞的敏感度。

DOI：
10.1016/0042-6989(82)90102-x
发表时间：
1982
期刊：
Vision research
影响因子：
1.8
作者：
Castańo,JA;Sperling,HG
通讯作者：
Sperling,HG

Detection of a light-sensitive pool of cGMP in goldfish cone color receptors by immunocytochemistry.

通过免疫细胞化学检测金鱼锥体颜色受体中 cGMP 的光敏池。

DOI：
10.1177/31.11.6311894
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
0
作者：
Ortez,RA;Tamayo,A;Johnson,C;Sperling,HG
通讯作者：
Sperling,HG

Color vision following intense green light exposure: data and a model.

强绿光照射后的色觉：数据和模型。