TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
批准号:
3252426
负责人:
LESTER G SULTATOS
金额:
$14.56万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-08-01 至 1995-08-31
关键词:
agriculture autoradiography biotransformation brain metabolism cytochrome P450 detoxification environmental toxicology enzyme activity enzyme substrate esterase glutathione high performance liquid chromatography human tissue immunochemistry laboratory mouse laboratory rat liver metabolism microsomes microspectrophotometry organophosphorus insecticide pesticide interaction phenobarbital radiotracer rifamycins serum thin layer chromatography toxicant interaction toxin metabolism
中文摘要
本建议的总体目标是提供信息,
这将有助于确定
个体可能异常敏感(或抵抗)
农药的毒性作用。 该项目将侧重于
有机磷杀虫剂,因为它们代表了
作为杀虫剂使用的化学物质,因为它们具有潜在的
对人体有严重的毒性作用。 研究将被导向
来描述那些影响哺乳动物
有机磷杀虫剂的生物转化反应,如
以及这些反应的后果。 预计在
这些研究将提供有用的信息,
有机磷杀虫剂对健康的危害,
可能与某些其他外来物质发生毒性相互作用
化学品 此外,这项研究将有助于
这些代谢系统的基本知识,
这些杀虫剂的生物转化 具体目标是
本研究的主要内容如下:1)继续调查
肝外激活的毒理学意义
有机硫磷杀虫剂2)调查为什么
谷胱甘肽依赖的解毒许多
二甲基取代的有机磷杀虫剂在体外
但不是在体内。3)确定和描述
影响肝脏和血清A-酯酶活性。 纳入本
目的将是动力学和物理化学的研究
“高活性”和“低活性”形式之间的差异
人血清A-酯酶。4)从生物化学的角度来描述
从小鼠肝微粒体中纯化的A-酯酶的形式,
实验室5)为了表征影响分泌的因素,
来自肝脏的A-酯酶。 这些研究还将侧重于
A-酯酶的分泌形式与那些
在肝微粒体中发现的形式。
英文摘要
The overall objective of this proposal is to provide information
which will aid in the identification of conditions in which
individuals might be unusually susceptible (or resistant) to the
toxic actions of pesticides. The project will focus on the
organophosphorus insecticides since they represent the major class
of chemicals used as insecticides, and since they have potential
for serious toxic effects in humans. Research will be directed
towards characterizing those factors which affect the mammalian
biotransformation reactions of organophosphorus insecticides, as
well as the consequences of such reactions. It is expected that
these studies will provide information useful in assessing the
health hazards of organophosphorus insecticides, as well as their
potential for toxic interactions with certain other foreign
chemicals. Additionally this proposed research will contribute to
the fundamental knowledge of those metabolic systems involved in
the biotransformation of these insecticides. The specific aims in
this research are as follows: 1) To continue to investigate the
toxicological significance of extrahepatic activation of certain
organothiophosphorus insecticides. 2) To investigate why
glutathione-dependent detoxification of numerous
dimethyl-substituted organophosphorus insecticides occurs in vitro
but not in vivo. 3) To identify and characterize factors which
affect hepatic and serum A-esterase activities. Included in this
aim will be the investigation of kinetic and physiochemical
differences between "high activity" and "low activity" forms of
human serum A-esterase. 4) To characterize biochemically the two
forms of A-esterase purified from mouse hepatic microsomes in this
laboratory. 5) To characterize the factors affecting secretion of
A-esterase(s) from liver. These studies will also focus on
comparisons of the secreted form(s) of A-esterase(s) with those
forms found in hepatic microsomes.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Insecticide Interactions With Acetylcholinesterase
-
批准号:7119627
-
项目类别:
-
资助金额:$28.85万
-
财政年份:2005
-
负责人:LESTER G SULTATOS
-
依托单位:
Insecticide Interactions With Acetylcholinesterase
-
批准号:6965782
-
项目类别:
-
资助金额:$30.07万
-
财政年份:2005
-
负责人:LESTER G SULTATOS
-
依托单位:
Insecticide Interactions With Acetylcholinesterase
-
批准号:7276733
-
项目类别:
-
资助金额:$28.01万
-
财政年份:2005
-
负责人:LESTER G SULTATOS
-
依托单位:
ALCOHOL-INDUCED LIVER DAMAGE: ROLE OF XANTHINE OXIDASE
-
批准号:3445338
-
项目类别:
-
资助金额:$6.47万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252430
-
项目类别:
-
资助金额:$13.03万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252428
-
项目类别:
-
资助金额:$8.85万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:2153650
-
项目类别:
-
资助金额:$16.17万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252432
-
项目类别:
-
资助金额:$6.55万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
ALCOHOL-INDUCED LIVER DAMAGE: ROLE OF XANTHINE OXIDASE
-
批准号:3445339
-
项目类别:
-
资助金额:$5.94万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252429
-
项目类别:
-
资助金额:$12.76万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252423
-
项目类别:
-
资助金额:$13.54万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252431
-
项目类别:
-
资助金额:$16.94万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3252427
-
项目类别:
-
资助金额:$1.53万
-
财政年份:1986
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3250719
-
项目类别:
-
资助金额:$1.51万
-
财政年份:1985
-
负责人:LESTER G SULTATOS
-
依托单位:
ALCOHOL-INDUCED LIVER DAMAGE: ROLE OF XANTHINE OXIDASE
-
批准号:3445203
-
项目类别:
-
资助金额:$5.02万
-
财政年份:1985
-
负责人:LESTER G SULTATOS
-
依托单位:
TOXICOLOGICAL SIGNIFICANCE OF PESTICIDE METABOLISM
-
批准号:3250718
-
项目类别:
-
资助金额:$8.96万
-
财政年份:1985
-
负责人:LESTER G SULTATOS
-
依托单位:
海外基金