DEVELOPMENTAL STUDIES OF THE VISUAL SYSTEM

视觉系统的发育研究

• 批准号: 3255896
• 负责人: RONALD E KALIL
• 金额: $ 12.69万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3255896
• 关键词: amblyopia; autoradiography; dendrites; electron microscopy; histochemistry /cytochemistry; immunochemistry; lateral geniculate body; mature animal; microscopy; neuroanatomy; neurochemistry; neurons; neurotransmitters; newborn animals; synapses; visual cortex; visual deprivation; visual pathways

The research proposed here is designed to clarify certain aspects of the structure and development of the retino-geniculo-cortical pathway in the cat. Four sets of experiments are planned: 1) Possible neurotransmitters in the retinogeniculate pathway will be investigated using light and electron microscopic immunohistochemistry and transmitter-specific retrograde labeling; 2) The effects of impulse blockade on the morphological development of retinogeniculate synapses will be studied in kittens that are reared with intraocular injections of tetrodotoxin; 3) Golgi methods will be used to reveal the effects of monocular deprivation and deafferentation on the dendritic development of neurons in the dorsal lateral geniculate nucleus (LGN); 4) The projection of the LGN to the lateral suprasylvian visual area in newborn and early postnatal kittens will be mapped using horseradish peroxidase (HRP) retrograde tracing methods, and it will be determined in adult cats that have had visual cortex removed at birth whether LGN relay cells that project to the lateral suprasylvian visual area receive direct synaptic input from the retina. The long-term objective of this research is to improve understanding of some of the mechanisms underlying the effects of visual deprivation and amblyopia, and in addition to shed some light on the modes of reorganization employed by the visual system to compensate for damage. These goals are approached by studying normal structure and development in concert with experiments designed to measure the direct effects of abnormal visual experience or brain injury.

这里提出的研究旨在澄清某些方面的 视网膜-膝状体-皮质通路的结构和发育 目录 计划进行四组实验：1）可能的神经递质 在视网膜神经通路中的作用将使用光和 电镜免疫组化和透射特异性 逆行标记; 2）脉冲阻滞对 将研究视网膜神经突触的形态发育 通过眼内注射河豚毒素饲养的小猫; 3） 高尔基方法将被用来揭示单眼剥夺的影响 和去传入对背侧神经元树突发育的影响 外侧膝状体核（LGN）： 新生和出生后早期幼猫外侧上裂视区 将使用辣根过氧化物酶（HRP）逆行追踪绘制 方法，并将在成年猫中确定， 出生时移除的皮质是否LGN中继细胞投射到外侧 外侧裂上视区接收来自视网膜的直接突触输入。 这项研究的长期目标是提高对 视觉剥夺影响的一些潜在机制， 弱视，除了阐明一些模式， 视觉系统用来补偿损害的重组。 这些目标是通过研究正常的结构和发展， 与旨在测量异常的直接影响的实验一致 视觉体验或脑损伤。