CELL BIOLOGY OF THE RETINA

视网膜的细胞生物学

• 批准号: 3255920
• 负责人: ELIO S RAVIOLA
• 金额: $ 23.53万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-08-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3255920
• 关键词: Macaca; action potentials; amacrine cells; cell biology; electron microscopy; electrophysiology; fluorescence; freeze etching; laboratory rabbit; light adaptations; membrane reconstitution /synthesis; neural information processing; neuropharmacology; neurophysiology; neurotransmitters; retina; retinal bipolar neuron; rod cell; sequential perception; silver impregnation; synaptic vesicles; visual feedback; visual perception; visual photoreceptor

This application is concerned with combining ultrastructural and electrophysiological techniques in the study of the neural interactions in the mammalian retina. It consists of three parts: i) We want to record from photoreceptors, horizontal and bipolar cells in slices and cell clumps isolated from the rabbit retina and to analyze their connections with the electron microscope. Purpose of these experiments is to understand the physiology of the various types of bipolar cells and the mechanisms underlying their response properties. ii) We want to record from the neurons of the macaque monkey retina maintained in a superfused eyecup preparation and identify them by intracellular injection of visible markers. Purpose of these experiments is to investigate the functional connectivity of a retina very similar to the human one. iii) By combining rapid freezing at 4 degree K , freeze-fracturing, ultrastructural tracer techniques and electrophysiology, we wish to analyze the mechanism of transmitter release and synaptic vesicle membrane recycling in solitary photoreceptor cells from the rabbit retina. Long-term objective of this application is to discover the mechanisms by which the retina processes visual information. This sort of studies are fundamental to the understanding and rational treatment of the disorders of the retina and nervous system in general.

本申请涉及将超微结构和 神经电生理学技术 哺乳动物视网膜中的相互作用。 它包括三个部分： i）我们希望从水平和双极光感受器记录 从兔视网膜分离的切片和细胞团中的细胞， 用电子显微镜分析它们之间的联系 这些实验的目的是了解 各种类型的双极细胞及其机制 它们的响应特性。 ii）我们想从神经元记录 猕猴的视网膜保持在一个灌流眼杯 制备并通过细胞内注射可见的 标记。 这些实验的目的是研究 视网膜的功能连接与人类非常相似。 iii）通过结合在4 ° K下的快速冷冻，冷冻断裂， 超微结构示踪技术和电生理学，我们希望 分析神经递质释放和突触传递的机制， 囊泡膜再循环在孤立的感光细胞， 兔子的视网膜 本申请的长期目标是 发现视网膜处理视觉的机制 信息. 这类研究对于 认识和合理治疗的障碍， 视网膜和神经系统。