ELECTRON MICROSCOPE JEOL JEM-1200EX

五位经验丰富的电子显微镜专家 本局提交一份更换两部过时电子产品的建议书 显微镜和一种新仪器。 所有的调查人员都严重依赖于电子显微镜在他们的 研究活动。这些研究包括： 1.哺乳动物视网膜对视觉信息的处理： 视网膜神经元被微电极穿透，它们的光 响应被记录，并且在细胞内递送之后 超微结构示踪剂，对其突触联系进行了研究 电子显微镜。它们各自产生的电信号 因此，单个细胞与其输入和输出相关。 2.缝隙连接和紧密连接的分子结构： 电子显微镜用于纯度的形态分析 在连接准备中，定位连接表位在 使用免疫细胞化学进行原位检测，最后结合 X射线衍射法测定GAP的分子结构 原子分辨率的结点。 3.细胞外基质在形态发生中的作用 电子显微镜被用来分析相互作用 细胞骨架与细胞外基质及细胞外基质对上皮细胞的诱导 间充质转化。 4.胃粘膜损伤和修复的过程： 用电子显微镜观察修复机制。 损伤和有利于实验动作的效果 修复过程。 调查人员目前使用的是两名15岁的儿童 需要持续维修且故障时间长的显微镜 周期；它们将完全停止的时刻即将到来。 他们想用一种新的、万能的、 透射电子显微镜，这结合了常规的高 快速获取显微照片的分辨率性能 在低倍率下。

Five experienced electron microscopists belonging to the same department submit a proposal to replace two outdated electron microscopes with a new instrument. All investigators heavily rely upon electron microscopy in their research activities. These include studies on: 1. Processing of visual information by the mammalian retina: retinal neurons are penetrated by microelectrodes, their light responses are recorded, and, after intracellular delivery of an ultrastructural tracer, their synaptic connections are studied with the electron microscope. The electric signals generated by each individual cell are thus correlated to its inputs and outputs. 2. Molecular structure of gap junctions and tight junctions: the electron microscope is used in a morphological assay for the purity of the junctional preparations, to localize junctional epitopes in situ using immunocytochemistry and, finally, in combination with X-ray diffraction, to determine the molecular structure of gap junctions to atomic resolution. 3. Role of extracellular matrix (ECM) in morphogenesis: the electron microscope is used to analyze the interaction of the cytoskeleton with ECM and the induction by ECM of epithelial- mesenchymal transformation. 4. Process of damage and repair of the gastric mucosa: the electron microscope is used to study the mechanism of repair after injury and the effects of experimental maneuvers that favor the restoration process. The investigators are presently using two fifteen year old microscopes that need continuous repairs and have long breakdown periods; the moment is near that they will come to a complete halt. They would like to substitute for them a new, all-purpose, transmission electron microscope, that combines a routine high resolution performance with great speed in obtaining micrographs at low magnification.