ELECTRON MICROSCOPE JEOL JEM-1200EX

电子显微镜 JEOL JEM-1200EX

• 批准号: 3519903
• 负责人: ELIO S RAVIOLA
• 金额: $ 20.1万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-11 至 1989-01-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519903
• 关键词: biomedical equipment purchase; cell biology; electron microscopy; immunocytochemistry; molecular biology

Five experienced electron microscopists belonging to the same department submit a proposal to replace two outdated electron microscopes with a new instrument. All investigators heavily rely upon electron microscopy in their research activities. These include studies on: 1. Processing of visual information by the mammalian retina: retinal neurons are penetrated by microelectrodes, their light responses are recorded, and, after intracellular delivery of an ultrastructural tracer, their synaptic connections are studied with the electron microscope. The electric signals generated by each individual cell are thus correlated to its inputs and outputs. 2. Molecular structure of gap junctions and tight junctions: the electron microscope is used in a morphological assay for the purity of the junctional preparations, to localize junctional epitopes in situ using immunocytochemistry and, finally, in combination with X-ray diffraction, to determine the molecular structure of gap junctions to atomic resolution. 3. Role of extracellular matrix (ECM) in morphogenesis: the electron microscope is used to analyze the interaction of the cytoskeleton with ECM and the induction by ECM of epithelial- mesenchymal transformation. 4. Process of damage and repair of the gastric mucosa: the electron microscope is used to study the mechanism of repair after injury and the effects of experimental maneuvers that favor the restoration process. The investigators are presently using two fifteen year old microscopes that need continuous repairs and have long breakdown periods; the moment is near that they will come to a complete halt. They would like to substitute for them a new, all-purpose, transmission electron microscope, that combines a routine high resolution performance with great speed in obtaining micrographs at low magnification.

五个经验丰富的电子显微镜属于同一个 运输署建议更换两部过时电子 显微镜与新仪器 所有的研究者都严重依赖电子显微镜在他们的研究中。 研究活动。 这些研究包括： 1. 哺乳动物视网膜对视觉信息的处理： 视网膜神经元被微电极穿透， 记录反应，并且在细胞内递送一种 超微结构示踪剂，它们的突触连接进行了研究， 电子显微镜。 每一个产生的电信号 因此，各个单元与其输入和输出相关。 2. 缝隙连接和紧密连接的分子结构 电子显微镜用于纯度的形态学测定 的连接制备，以定位连接表位， 原位使用免疫细胞化学，最后，结合 X射线衍射，确定GAP的分子结构 连接到原子分辨率。 3.细胞外基质（ECM）在形态发生中的作用： 电子显微镜是用来分析的相互作用， 细胞骨架与细胞外基质的关系以及细胞外基质对上皮细胞的诱导作用。 间质转化 4.胃粘膜的损伤与修复过程 用电镜观察修复机制 伤害和实验演习的影响，有利于 恢复过程。 调查人员目前正在使用两名15岁的 需要连续维修和长期故障的显微镜 时间;那一刻是近了，他们将完全停止。 他们想用一种新的，通用的， 透射电子显微镜，它结合了常规的高 分辨率性能，获得显微照片的速度快 低倍放大。