A multi-application microarray robot for agri-food research, industrial biotechnology and on-chip manufacturing

用于农业食品研究、工业生物技术和芯片制造的多应用微阵列机器人

• 批准号: BB/S019804/1
• 负责人: William Willats
• 金额: $ 42.64万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS019804%2F1
• 关键词: multi; application; microarray; robot; agri

We are seeking funding to acquire a microarray robot for bioscience research. These machines are in effect printers that instead of ink, print biological samples in the form of spots on a surface such as a slide or membrane. The power of microarrays and their value to life science research is immense because entire libraries of molecules from complex biological systems can be represented in the form of tens of thousands of spots in an area no bigger than a postage stamp. With these arrayed libraries, we can explore interactions between molecules, test for biological activities, screen for new drugs and discover new enzymes. This technology was first applied to nucleic acids (DNA and RNA), but microarrays are now used for research into proteins, carbohydrates, lipids and small molecules. The technology is especially powerful for deciphering interaction networks between genes and proteins and for large scale screening of antibodies, enzymes and other bio-molecules. Furthermore, the latest generation of microarray robots are also advanced manufacturing devices capable of fabricating sensors and assays and can be used for performing multiplexed (many simultaneously) microscale experiments in-situ within the controlled environment of the machine. The equipment that we are requesting will be custom manufactured with bespoke features that greatly enhance its capacity and versatility, by a globally-leading UK company. By embedding this equipment within a new Biosystems Engineering unit at Newcastle University, we will create a truly world-class microarray technology hub that will accelerate research in food security, bioenergy and industrial biotechnology and health bioscience. By working with locally based but globally active industry partners we will use this equipment to drive translational research towards commercialisation. The equipment will also ensure that a new generation of UK students will have access to state-of-the-art technology, bringing a lasting legacy of research benefits.

我们正在寻求资金，以获得用于生物科学研究的微阵列机器人。这些机器实际上是一种打印机，它不是墨水，而是在玻片或薄膜等表面上以斑点的形式打印生物样本。微阵列的力量及其对生命科学研究的价值是巨大的，因为来自复杂生物系统的整个分子库可以在不超过一张邮票大小的区域内以数万个点的形式表示。有了这些排列的文库，我们可以探索分子之间的相互作用，测试生物活性，筛选新药和发现新的酶。这项技术最初应用于核酸(DNA和RNA)，但现在微阵列用于蛋白质、碳水化合物、脂肪和小分子的研究。这项技术在破译基因和蛋白质之间的相互作用网络以及大规模筛选抗体、酶和其他生物分子方面尤其强大。此外，最新一代的微阵列机器人也是先进的制造设备，能够制造传感器和分析，并可用于在机器的受控环境中原位执行多路(多个同时)微型实验。我们要求的设备将由一家全球领先的英国公司定制制造，具有定制的功能，大大增强其容量和多功能性。通过将该设备嵌入纽卡斯尔大学的一个新的生物系统工程部门，我们将创建一个真正的世界级微阵列技术中心，将加快食品安全、生物能源、工业生物技术和健康生物科学的研究。通过与当地但全球活跃的行业合作伙伴合作，我们将使用这种设备推动翻译研究走向商业化。这些设备还将确保新一代英国学生能够接触到最先进的技术，带来持久的研究成果。

项目成果

Analysis of glycans in a Burnt-on/Baked-on (BoBo) model food soil using Microarray Polymer Profiling (MAPP) and immunofluorescence microscopy.

使用微阵列聚合物分析 (MAPP) 和免疫荧光显微镜分析烧焦/烘烤 (BoBo) 模型食物土壤中的聚糖。

• DOI: 10.1016/j.foodchem.2022.135379
• 发表时间: 2023
• 期刊: Food chemistry
• 影响因子: 8.8
• 作者: Bakshani CR

Combinatorial Glycomic Analyses to Direct CAZyme Discovery for the Tailored Degradation of Canola Meal Non-Starch Dietary Polysaccharides.

• DOI: 10.3390/microorganisms8121888
• 发表时间: 2020-11-29
• 期刊: Microorganisms
• 影响因子: 4.5
• 作者: Low KE;Xing X;Moote PE;Inglis GD;Venketachalam S;Hahn MG;King ML;Tétard-Jones CY;Jones DR;Willats WGT;Slominski BA;Abbott DW
• 通讯作者: Abbott DW

Diatom fucan polysaccharide precipitates carbon during algal blooms.

• DOI: 10.1038/s41467-021-21009-6
• 发表时间: 2021-02-19
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Vidal-Melgosa S;Sichert A;Francis TB;Bartosik D;Niggemann J;Wichels A;Willats WGT;Fuchs BM;Teeling H;Becher D;Schweder T;Amann R;Hehemann JH
• 通讯作者: Hehemann JH

Ancient origin of fucosylated xyloglucan in charophycean green algae.

• DOI: 10.1038/s42003-021-02277-w
• 发表时间: 2021-06-17
• 期刊: Communications biology
• 影响因子: 5.9
• 作者: Mikkelsen MD;Harholt J;Westereng B;Domozych D;Fry SC;Johansen IE;Fangel JU;Łężyk M;Feng T;Nancke L;Mikkelsen JD;Willats WGT;Ulvskov P
• 通讯作者: Ulvskov P

Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.

• DOI: 10.3389/fpls.2022.785902
• 发表时间: 2022
• 期刊: FRONTIERS IN PLANT SCIENCE
• 影响因子: 5.6
• 作者: Salmean, Armando A.;Willats, William George Tycho;Ribeiro, Sofia;Andersen, Thorbjorn Joest;Ellegaard, Marianne
• 通讯作者: Ellegaard, Marianne