WFUSM Macaca fascicularis SPF Breeding Colony

描述（由申请人提供）：人们普遍认为，非人灵长类动物是生物医学研究的宝贵资源。食蟹猴（Macaca fascicularis）是两种最广泛使用的猕猴物种之一，在传染病和免疫疾病、糖尿病和心血管疾病、妇女健康、骨质疏松症和生殖道癌症方面具有生物医学应用。我们建议扩大现有的无特定病原体（SPF）食蟹猴示范群，作为NIH受资助者的国家资源。这项提案涉及维克森林大学医学院（WFUSM）和华盛顿国家灵长类动物研究中心（WaNPRC）之间的合作。WFUSM将提供基础设施，菌落管理，遗传监测和慢性疾病建模（心血管，骨骼，代谢和肿瘤疾病）的专业知识，而WaNPRC则提供病毒学，免疫学和SPF育种方面的专业知识。确定病原体状态的国内繁殖群的建立将提供许多优势，包括直接获得源动物和隔离由于政治动荡、恐怖主义、运输延迟和国际检疫而导致的潜在供应中断。殖民地将位于自然灾害（洪水、飓风、龙卷风和地震）风险较低的地区。养殖地将产生对结核分枝杆菌（TB）、猿猴逆转录病毒（SRV）、猿猴T细胞营养病毒（STLV）、猿猴免疫缺陷病毒（SIV）和猕猴疱疹病毒1型（CHV-1）呈阴性的动物。这种SPF状态将允许后代动物更广泛的研究用途，并通过消除潜在的致命病原体CHV-1的潜在人畜共患传播，为研究人员提供显著的益处。除了SPF状态外，猴子还将被喂食定制的不含杀虫剂和其他内分泌干扰物的饮食。大多数商业猴子咀嚼物含有雌激素;这些结合雌激素受体并具有许多生物作用。作为殖民地研究计划的一部分，为了更好地了解饮食如何影响胎儿编程和后来的慢性疾病风险，一半的猴子将被喂食不含孕酮的饮食，另一半则被喂食含有孕酮的饮食，其水平与商业食物中发现的水平相似。这些条件将为NIH赞助的生物医学研究提供SPF和饮食定义的食蟹猴的独特资源。

DESCRIPTION (provided by applicant): It is generally recognized that nonhuman primates comprise an invaluable resource for biomedical research. The cynomolgus monkey (Macaca fascicularis) is one of the two most widely used macaque species, with biomedical applications in infectious disease and immune disorders, diabetes and cardiovascular disease, women's health, osteoporosis, and reproductive tract cancer. We proposed to expand an existing demonstration colony of specific pathogen free (SPF) cynomolgus monkeys to function as a national resource for NIH grantees. This proposal involves a collaboration between Wake Forest University School of Medicine (WFUSM) and the Washington National Primate Research Center (WaNPRC). WFUSM will provide infrastructure, colony management, genetic monitoring, and expertise in chronic disease modeling (cardiovascular, skeletal, metabolic, and oncologic disorders), while the WaNPRC contributes expertise in virology, immunology, and SPF breeding. Establishment of a domestic breeding colony of defined pathogen status will provide many advantages, including direct access to source animals and insulation from potential interruptions of supply due to political unrest, terrorism, shipping delays, and international quarantine. The colony will be located in a region at low risk of natural disasters (flood, hurricane, tornado, and earthquake).The breeding colony will produce animals negative for Mycobacterium tuberculosis (TB), Simian Retrovirus(SRV), Simian T-Lymphotrophic Virus (STLV), Simian Immunodeficiency Virus (SIV), and Cercopithecine Herpes Virus 1 (CHV-1). This SPF status will allow a broader range of research use for progeny animals, and provides a significant benefit to research staff by removal of latent zoonotic transmission of CHV-1, a potentially lethal pathogen. In addition to their SPF status, the monkeys will be fed a custom-prepared diet free of pesticides and other endocrine disrupters. Most commercial monkey chows contain isoflavones; these bind to estrogen receptors and have numerous biological actions. As part of the colony research program to understand better how diet many influence fetal programming and later chronic disease risk, half of the monkeys will be fed an isoflavone-free diet and half fed a diet with isoflavones at levels similar to those found in commercial chow. These conditions will provide a unique resource of both SPF and diet-defined Macaca fascicularis available for NIH-sponsored biomedical research.