WFUSM Macaca fascicularis SPF Breeding Colony

描述（由申请人提供）：人们普遍认为非人类灵长类动物是生物医学研究的宝贵资源。食蟹猴（Macaca fascicularis）是两种应用最广泛的猕猴物种之一，在传染病和免疫紊乱、糖尿病和心血管疾病、妇女健康、骨质疏松症和生殖道癌症等领域具有生物医学应用。我们提议扩大现有的无特定病原体 (SPF) 食蟹猴示范群落，使其成为 NIH 受资助者的国家资源。该提案涉及维克森林大学医学院 (WFUSM) 和华盛顿国家灵长类动物研究中心 (WaNPRC) 之间的合作。 WFUSM 将提供基础设施、群体管理、遗传监测和慢性疾病模型（心血管、骨骼、代谢和肿瘤疾病）方面的专业知识，而 WaNPRC 则提供病毒学、免疫学和 SPF 育种方面的专业知识。建立具有明确病原体状况的国内繁殖群将提供许多优势，包括直接获得来源动物以及避免由于政治动荡、恐怖主义、运输延误和国际检疫而可能造成的供应中断。该群体将位于自然灾害（洪水、飓风、龙卷风和地震）风险较低的地区。繁殖群体将生产出对结核分枝杆菌（TB）、猿逆转录病毒（SRV）、猿T淋巴细胞病毒（STLV）、猿免疫缺陷病毒（SIV）和猴疱疹病毒1（CHV-1）呈阴性的动物。这种 SPF 状态将允许对子代动物进行更广泛的研究用途，并通过消除潜在的人畜共患 CHV-1（一种潜在的致命病原体）的潜在人畜共患传播，为研究人员提供显着的好处。除了 SPF 状态外，这些猴子还将接受不含农药和其他内分泌干扰物的定制饮食。大多数商业猴粮都含有异黄酮。它们与雌激素受体结合并具有多种生物作用。作为为了更好地了解饮食如何影响胎儿发育和后来的慢性疾病风险的群体研究计划的一部分，一半的猴子将被喂食不含异黄酮的饮食，另一半则喂食含有异黄酮的饮食，其含量与商业饲料中的异黄酮水平相似。这些条件将为 NIH 资助的生物医学研究提供 SPF 和饮食定义的食蟹猴的独特资源。

DESCRIPTION (provided by applicant): It is generally recognized that nonhuman primates comprise an invaluable resource for biomedical research. The cynomolgus monkey (Macaca fascicularis) is one of the two most widely used macaque species, with biomedical applications in infectious disease and immune disorders, diabetes and cardiovascular disease, women's health, osteoporosis, and reproductive tract cancer. We proposed to expand an existing demonstration colony of specific pathogen free (SPF) cynomolgus monkeys to function as a national resource for NIH grantees. This proposal involves a collaboration between Wake Forest University School of Medicine (WFUSM) and the Washington National Primate Research Center (WaNPRC). WFUSM will provide infrastructure, colony management, genetic monitoring, and expertise in chronic disease modeling (cardiovascular, skeletal, metabolic, and oncologic disorders), while the WaNPRC contributes expertise in virology, immunology, and SPF breeding. Establishment of a domestic breeding colony of defined pathogen status will provide many advantages, including direct access to source animals and insulation from potential interruptions of supply due to political unrest, terrorism, shipping delays, and international quarantine. The colony will be located in a region at low risk of natural disasters (flood, hurricane, tornado, and earthquake).The breeding colony will produce animals negative for Mycobacterium tuberculosis (TB), Simian Retrovirus(SRV), Simian T-Lymphotrophic Virus (STLV), Simian Immunodeficiency Virus (SIV), and Cercopithecine Herpes Virus 1 (CHV-1). This SPF status will allow a broader range of research use for progeny animals, and provides a significant benefit to research staff by removal of latent zoonotic transmission of CHV-1, a potentially lethal pathogen. In addition to their SPF status, the monkeys will be fed a custom-prepared diet free of pesticides and other endocrine disrupters. Most commercial monkey chows contain isoflavones; these bind to estrogen receptors and have numerous biological actions. As part of the colony research program to understand better how diet many influence fetal programming and later chronic disease risk, half of the monkeys will be fed an isoflavone-free diet and half fed a diet with isoflavones at levels similar to those found in commercial chow. These conditions will provide a unique resource of both SPF and diet-defined Macaca fascicularis available for NIH-sponsored biomedical research.