WFUSM Macaca fascicularis SPF Breeding Colony

描述（由申请人提供）：人们普遍认为非人类灵长类动物是生物医学研究的宝贵资源。食蟹猴（Macaca fascicularis）是两种应用最广泛的猕猴之一，在传染病和免疫疾病、糖尿病和心血管疾病、妇女健康、骨质疏松症和生殖道癌等生物医学领域有广泛的应用。我们建议扩大一个现有的无特定病原体（SPF）食蟹猴示范菌落，作为NIH资助项目的国家资源。该提案涉及维克森林大学医学院（WFUSM）和华盛顿国家灵长类动物研究中心（WaNPRC）之间的合作。WFUSM将提供基础设施、菌落管理、遗传监测和慢性病建模（心血管、骨骼、代谢和肿瘤疾病）方面的专业知识，WaNPRC将提供病毒学、免疫学和SPF育种方面的专业知识。建立一个明确病原体状态的国内繁殖群体将提供许多优势，包括直接接触源动物和避免因政治动荡、恐怖主义、航运延误和国际检疫而导致的潜在供应中断。该殖民地将位于自然灾害（洪水、飓风、龙卷风和地震）风险较低的地区。该繁殖菌落将产生对结核分枝杆菌（TB）、猴逆转录病毒（SRV）、猴t淋巴营养病毒（STLV）、猴免疫缺陷病毒（SIV）和猴颈猿疱疹病毒1 （CHV-1）呈阴性反应的动物。这种SPF状态将允许后代动物更广泛的研究用途，并通过消除潜在致命病原体CHV-1的潜在人畜共患传播，为研究人员提供重大利益。除了它们的SPF值外，这些猴子还将被喂食不含杀虫剂和其他内分泌干扰物的定制饮食。大多数商业猴粮都含有异黄酮；它们与雌激素受体结合并具有许多生物学作用。为了更好地了解饮食如何影响胎儿发育和后来的慢性疾病风险，作为群体研究项目的一部分，一半的猴子将被喂食不含异黄酮的食物，另一半喂食含有与商业饲料中异黄酮含量相似的食物。这些条件将为美国国立卫生研究院资助的生物医学研究提供独特的SPF和饮食定义的Macaca Macaca fascularis资源。

DESCRIPTION (provided by applicant): It is generally recognized that nonhuman primates comprise an invaluable resource for biomedical research. The cynomolgus monkey (Macaca fascicularis) is one of the two most widely used macaque species, with biomedical applications in infectious disease and immune disorders, diabetes and cardiovascular disease, women's health, osteoporosis, and reproductive tract cancer. We proposed to expand an existing demonstration colony of specific pathogen free (SPF) cynomolgus monkeys to function as a national resource for NIH grantees. This proposal involves a collaboration between Wake Forest University School of Medicine (WFUSM) and the Washington National Primate Research Center (WaNPRC). WFUSM will provide infrastructure, colony management, genetic monitoring, and expertise in chronic disease modeling (cardiovascular, skeletal, metabolic, and oncologic disorders), while the WaNPRC contributes expertise in virology, immunology, and SPF breeding. Establishment of a domestic breeding colony of defined pathogen status will provide many advantages, including direct access to source animals and insulation from potential interruptions of supply due to political unrest, terrorism, shipping delays, and international quarantine. The colony will be located in a region at low risk of natural disasters (flood, hurricane, tornado, and earthquake).The breeding colony will produce animals negative for Mycobacterium tuberculosis (TB), Simian Retrovirus(SRV), Simian T-Lymphotrophic Virus (STLV), Simian Immunodeficiency Virus (SIV), and Cercopithecine Herpes Virus 1 (CHV-1). This SPF status will allow a broader range of research use for progeny animals, and provides a significant benefit to research staff by removal of latent zoonotic transmission of CHV-1, a potentially lethal pathogen. In addition to their SPF status, the monkeys will be fed a custom-prepared diet free of pesticides and other endocrine disrupters. Most commercial monkey chows contain isoflavones; these bind to estrogen receptors and have numerous biological actions. As part of the colony research program to understand better how diet many influence fetal programming and later chronic disease risk, half of the monkeys will be fed an isoflavone-free diet and half fed a diet with isoflavones at levels similar to those found in commercial chow. These conditions will provide a unique resource of both SPF and diet-defined Macaca fascicularis available for NIH-sponsored biomedical research.