ACTIVATION OF THE HEXOSE MONOPHOSPHATE SHUNT IN THE LENS

目前已知，人类老年性白内障经历了广泛的 氧化改变 这一点可以从以下几个方面得到证明： 白内障中富含二硫化物的高分子量蛋白质聚集体 眼镜. 氧化的来源尚未确定。 然而，在眼房水中发现H2O2水平升高 和人类白内障患者的透镜。 这表明H2O2及其 衍生物可能参与白内障的发生， 无法有效地解毒H2 O2。 我们建议调查： (1)透镜的H2O2解毒功能是否存在固有缺陷 机制，（2）是否存在与年龄相关的H2O2解毒损失， 特别是己糖单磷酸分流偶联系统，（3）是否 H2O2引起的损害可以识别，以及（4）是否有可能 增强透镜对H2O2的抵抗力。 生化分析和核分析 磁共振光谱学将在该提议中使用。 成功完成拟议的研究和示范， 可行性将使我们能够开发用于 治疗人老年性白内障。

It is now known that human senile cataracts have undergone extensive oxidative alteration. This is evidenced by the accumulation of disulfide-rich high molecular weight protein aggregates in the cataractous lenses. The source of oxidation has not yet been definitively identified. Elevated H2O2 levels, however, have been discovered in the aqueous humor and the lens of human cataract patients. This suggests that H2O2 and its derivatives may be involved in the cataractogenesis and that the cataracts are incapable of detoxifying H2O2 efficiently. We propose to investigate: (1) whether there is an inherent weakness in the lens's H2O2 detoxifying mechanism, (2) whether there is an age-related loss of H2O2 detoxification, particularly the hexose monophosphate shunt-coupled system, (3) whether H2O2-induced damage can be identified, and (4) whether it is possible to augment the lens's resistance to H2O2. Both biochemical assays and nuclear magnetic resonance spectroscopy will be employed in this proposal. Successful completion of the proposed studies and demonstration of feasibility will enable us to develop anti-cataract agent(s) for the treatment of human senile cataracts.