REGIONAL NMR MULTINUCLEAR STUDIES OF OCULAR TISSUES

本申请的主要目的是应用新型磁性材料。 共振成像技术检查水运输的 角膜和透镜的生物学特性。 具体目的是：（1）分析角膜在无氧条件下的水分转运 使用T2造影剂H217 O应力;和（2）成像水自身， 扩散、自由水和束缚水的交换以及 代谢应激镜片。 我们将用兔子作实验动物。 实验模型 包括急性半乳糖血症性白内障和角膜缺氧。的 对角膜周围的水运输的影响因素如流泪， 角膜缘循环和房水流量通过药物测试 改变这些因素。这些都是简单而有效的模型，可以 用于开发先进的眼部MRI技术。 拟议的研究对于建立基于MRI的 眼前节疾病的功能评估及其治疗。 结果将提供有用的信息：（1）正常和病理 角膜与水的相互作用-在评估 例如，干眼、伤口愈合和隐形透镜佩戴;和（2）水 在代谢过程中透镜的不同区域中的运动 白内障发生-重要的是在进一步了解 白内障形成过程中的生物物理变化和疗效测试 抗白内障治疗。

The principal objective of this application is to apply novel magnetic resonance imaging techniques to the examination of water transport by the cornea and the lens in vivo. The specific aims are: (1) analyze corneal water transport under anaerobic stress using a T2 contrast agent H217O; and (2) image water self- diffusion, free- and bound-water interchange, and waterbulk-flow in metabolically stressed lenses. We will use rabbits as the experimental animal. The experimental models include acute galactosemic cataract and corneal oxygen deprivation. The effects on water transport of pericorneal factors such as lacrimation, limbal circulation and aqueous flow are tested by pharmaceutically altering these factors. These are simple yet effective models that can be used to develop advanced ocular MRI techniques. The proposed studies are essential to the establishment of MRI-based functional assessment of anterior segment diseases and their therapies. The results will provide useful information on (1) normal and pathological corneal interaction with water - important in the evaluation of, for example, dry eyes, wound-healing and contact lens wear; and (2) water movement in different regions of the lens during metabolic cataractogenesis - important in the further understanding of the biophysical changes during cataract formation and testing of the efficacy of anti-cataract therapy.