RESONANCE RAMAN STUDIES OF RHODOPSIN & RELATED PIGMENTS

视紫质的共振拉曼研究

• 批准号: 3257398
• 负责人: Robert Callender
• 金额: $ 16.35万
• 依托单位: CITY COLLEGE OF NEW YORK
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3257398
• 关键词: Raman spectrometry; Schiff bases; bacteriorhodopsins; chromophore; molecular rearrangement; photochemistry; protonation; rhodopsin; saltwater environment; stop flow technique

The overall goal of this research is to study the mechanism of light energy transduction in visual pigments and related pigments using resonance Raman spectroscopy. This technique is a very powerful one to probe the structure and changes in structure of the visual pigment retinal chromophore that accompany the early stages of vision. The ultimate goal is to understand from a molecular viewpoint how light energy is converted to what chemistry that is called visual transduction. Transduction processes are important problems in biology, and a molecular understanding of the visual system is an important step. Three main areas of study are envisioned. Experiments are planned to elucidate the nature of the chromophoric site. The structure of the primary photoproducts are to be measured so as to understand how light energy is converted to chemical energy. Lastly, dark intermediates and reaction pathways are to be probed to help elucidate how this chemical energy is used.

本研究的总体目标是研究光能的作用机理 使用共振拉曼在视色素和相关色素中的转导 谱 这项技术是一种非常强大的探测结构的技术 以及视色素视网膜发色团结构的变化， 伴随着视力的早期阶段。 最终目的是了解 从分子的角度来看，光能是如何转化为 叫做视觉传导。 转导过程很重要 生物学中的问题，以及对视觉系统的分子理解， 重要的一步。 设想了三个主要的研究领域。 实验 计划阐明发色位点的性质。 的 测量初级光产物的结构，以便 了解光能如何转化为化学能。 最后，黑暗 中间体和反应途径将被探测，以帮助阐明如何 这种化学能被使用。