REGULATION OF NEURAL PLASTICITY IN MATURING VISUAL CELLS

我们研究的长期目标是识别细胞和分子 未成熟皮质细胞对视觉具有独特敏感性的基础 在出生后的早期经历。认识关键时期 猫模型的可塑性可能有助于阐明神经机制 潜在性弱视先于失明。 利用1-去甲肾上腺素的增塑作用(1- NA)直接注入麻醉大鼠的再生视皮层 瘫痪的小猫，我们想探索一种新的策略，我们可以用它 迅速改变单个细胞的眼睛优势。为响应新的 表明突触后活动参与的证据- 问题中的依赖机制，可能是这些 视皮层内NA-B肾上腺素受体系统的作用机制 在患有慢性疾病的小猫中寻找。将进行另一项长期研究 洞察可能整合这两个B蛋白的可能的靶分子 肾上腺素受体和毒鼠碱受体依赖的过程 皮质可塑性的调节。我们还想进行一次彻底的 猫视皮层再生NA终末的研究 中枢神经系统轴突再生的广泛神经科学问题。 我们将在麻醉和瘫痪的1-NA灌流的大脑皮质中进行研究 小猫双眼反应变化的时间进程 在之前和期间的不同时间间隔的光学诱导斜视 以群体和单细胞为基础的感受野映射 (“在线”修改)，2)功能单元类型(简单、复杂和 具有末端停止性质的HyerComplex)，每个 记录的细胞及其与最近眼睛中心的关系 组织学上可见的优势斑块。在类似的 如上所述，我们将研究其增强塑性的效果 电刺激传递到蓝斑的NA细胞。 3)通过向受感染的皮质区域局部注入蝇油 在(没有时间重叠)输注心得安之前，我们将研究 是否表现出“蝇毒酚效应”取决于 NA-B肾上腺素受体系统的功能。4)我们将确认并 扩展我们的初步结果，即眼睛优势的转变 小猫单眼眼睑短暂缝合后，反复缝合 IP地址。注射Li2CO3。5)我们还将研究是否存在 注入-6-羟基多巴胺的真实再生NA终末 使用多学科方法对成年猫的视觉皮质进行研究。

The long-term goal of our study is to identify cellular and molecular bases for the unique sensitivity of immature cortical cells to visual experience early in postnatal life. Understanding the critical period plasticity in the cat model may help to elucidate neural mechanisms underlying amblyopia ex anopsia. Taking advantage of plasticity-enhancing effects of 1-noradrenaline (1- NA) directly infused into aplastic visual cortex of anesthetized and paralyzed kittens, we want to explore a new strategy with which we can quickly alter ocular dominance of individual cells. In response to new evidence which suggests the involvement of postsynaptic activity- dependent mechanisms in the matter, likely interactions among such the mechanisms and the NA-B adrenoreceptor system within visual cortex will be sought in chronic kittens. Another chronic study will be made to gain insight into possible target molecules which may integrate both B adrenoreceptor - and muscarinic receptor-dependent processes int eh regulation of cortical plasticity. We also want to carry out a thorough study on regenerative NA terminals in cat visual cortex, addressing a broad neuroscience question of regeneration of CNS axons. We will study in the 1-NA-infused cortex of anesthetized and paralyzed kittens 1) time courses of changes in binocularity response to optically induced squint at various time intervals before and during receptive-field mappings, as a population and on the single-cell basis ("on-line" modification), 2) functional cell types (simple, complex and hyercomplex with end-stopping properties), laminar location of every recorded cell, and its relation to the center of the nearest ocular dominance patches which are visualized histologically. In the similar context as above, we will study the plasticity-enhancing effect of electrical stimulation delivered to the NA cells in the locus coeruleus. 3) By locally infusing muscimol into a cortical area affected by a preceding (no temporal overlap) infusion with propranolol, we will study whether the expression of the "muscimol effects" is dependent on the function of the NA-B adrenoreceptor system. 4) We will confirm and extend our preliminary results that a shift in ocular dominance following brief monocular lid suture in kittens is blocked by repeated i.p. injections of Li2 CO3. 5) We will also study the presence of genuine regenerative NA terminals in the-6-hydroxydopamine-infused visual cortex of adult cats using a multidisciplinary approach.