Translating miRNA biomarker discovery into novel veterinary diagnostics to increase the efficiency of milk production

将 miRNA 生物标志物的发现转化为新型兽医诊断方法，以提高产奶效率

• 批准号: BB/T004037/1
• 负责人: Francesc Donadeu
• 金额: $ 50.79万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT004037%2F1
• 关键词: Translating; miRNA; biomarker; discovery; into

This study will address the long-standing problem of low fertility in dairy cows which through reduced milk production results in significant profit loss by UK and global industries. We will develop and commercialise a robust, affordable assay for early pregnancy detection to facilitate reproductive management of cows and increase milk production efficiency. Specifically, following on our previous discovery of early pregnancy biomarkers we will establish optimum conditions for miRNA quantification in blood and milk for accurately determining early pregnancy status in commercial cattle. We will then seek to develop and test a novel assay platform for direct and accurate detection of miRNAs in cattle samples using proprietary nucleic acid technology. Finally, we will launch a laboratory-based or pen-side assay through spin-out company creation or licensing to existing Diagnostics companies. We envision this will bring significant improvements to productivity and welfare of dairy cows.

这项研究将解决奶牛生育力低的长期问题，这一问题通过减少牛奶产量导致英国和全球工业的重大利润损失。我们将开发一种强大的、负担得起的早期怀孕检测方法并将其商业化，以促进奶牛的繁殖管理并提高牛奶生产效率。具体地说，根据我们之前发现的早孕生物标志物，我们将建立血液和乳汁中miRNA定量的最佳条件，以准确确定商品牛的早孕状态。然后，我们将寻求开发和测试一种新的检测平台，使用专有的核酸技术直接和准确地检测牛样本中的miRNAs。最后，我们将通过创建分拆公司或向现有的诊断公司发放许可证，推出基于实验室或笔端的分析。我们预计，这将显著提高奶牛的生产率和福利。

项目成果

Association between blood miR-26a levels following artificial insemination, and pregnancy outcome in dairy cattle.

• DOI: 10.1371/journal.pone.0289342
• 发表时间: 2023
• 期刊: PloS one
• 影响因子: 3.7

MiRNAs in milk can be used towards early prediction of mammary gland inflammation in cattle.

• DOI: 10.1038/s41598-022-09214-9
• 发表时间: 2022-03-24
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Tzelos T;Ho W;Charmana VI;Lee S;Donadeu FX
• 通讯作者: Donadeu FX