课题基金 / 基金详情

BIOLOGICAL OXIDATION MECHANISMS

BIOLOGICAL OXIDATION MECHANISMS
生物氧化机制
批准号：
3268236
负责人：
VINCENT MASSEY
金额：
$28.27万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
项目状态：
已结题
起止时间：
1978-09-01 至 1988-08-31
来源：
https://reporter.nih.gov/project-details/3268236
关键词：
Escherichia coli X ray crystallography aminoacid oxidase charge transfer complex chemical reaction cofactor electron transport enzyme model enzyme structure enzyme substrate complex flavins flavoproteins fluorescence spectrometry hydroxybenzoate hydroxylation lactate dehydrogenases oxidation oxygenases protonation radiotracer spectrometry

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中文摘要
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英文摘要
The main aim of the research project is an understanding of the chemical reactivity of flavin coenzymes and how this reactivity is used and controlled in biological systems. The methods of approach will be the study of specific enzymes representative of the various classes of flavoproteins and studies of model systems. Considerable use will be made of steady state kinetics coupled with rapid reaction kinetic studies, and of the technique of replacing the natural coenzymes of falvoproteins with synthetic flavins, in order to test hypothetical schemes and to probe the nature of the environment immediately around the flavin. The latter is particularly important in providing information about the active site region in proteins where the structure is not available from X-ray crystallography. We also plan to undertake the amino acid sequence of L-lactate oxidase in order to aid in the crystallographic determination of its three-dimensional structure.
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