BIOLOGICAL OXIDATION MECHANISMS

这个研究项目的主要目的是了解这种化学物质。 黄素辅酶的反应性以及如何利用这种反应性 在生物系统中控制的。方法将是 代表不同类别的特定酶的研究 黄素蛋白和模型系统的研究。将有相当大的用处 稳态动力学与快速反应动力学研究相结合，以及 用来代替假蛋白的天然辅酶的技术 合成黄素，以测试假设方案并探索 自然界中紧邻黄昏的环境。后者是 在提供有关活动站点的信息时尤其重要 蛋白质中X射线不能提供结构的区域 结晶学。我们还计划承担氨基酸序列 L-乳酸氧化物酶辅助结晶学测定黄曲霉毒素 它的三维结构。

The main aim of the research project is an understanding of the chemical reactivity of flavin coenzymes and how this reactivity is used and controlled in biological systems. The methods of approach will be the study of specific enzymes representative of the various classes of flavoproteins and studies of model systems. Considerable use will be made of steady state kinetics coupled with rapid reaction kinetic studies, and of the technique of replacing the natural coenzymes of falvoproteins with synthetic flavins, in order to test hypothetical schemes and to probe the nature of the environment immediately around the flavin. The latter is particularly important in providing information about the active site region in proteins where the structure is not available from X-ray crystallography. We also plan to undertake the amino acid sequence of L-lactate oxidase in order to aid in the crystallographic determination of its three-dimensional structure.