CELLULAR AND EMBRYONIC STUDIES ON CONGENITAL CATARACT

先天性白内障的细胞和胚胎研究

• 批准号: 3262826
• 负责人: Audray Ken Kay Harris
• 金额: $ 5.16万
• 依托单位: MEDICAL RES COUNCIL LAB OF MOLEC BIOL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3262826
• 关键词: autosomal dominant trait; cataract; cell transformation; cellular pathology; chromosome disorders; congenital vision disorder; embryo /fetus; gene expression; genetic manipulation; genetic markers; genetic strain; histogenesis; histopathology; hybrid cells; intermediate filaments; laboratory mouse; lens proteins; mammalian embryology; mature animal; microfilaments; microtubules; tissue /cell culture; tissue mosaicism

Congenital cataract is both an ophthalmologic and social problem. The role of hereditary has shown it forms 8.25% of all congenital cataracts. A genetic analysis in humans is not possible due to generation times and small numbers of progeny. Mouse congenital cataract mutants Cat-Fraser (Cat-Fr) and Lop provide an excellent system to study inheritance of cataract. They also provide a method to undertake in vivo and in vitro research on the growth, replication and gene expression in lenses of the cataractous mice. Breeding experiments are presently in progress to assign a specific chromosome for the dominant inherited Cat-Fr mouse. Chromosome 10 has already been mapped for the Lop cataract. Experimental chimeras formed from the aggregation of preimplantation embryos of cataractous and non-cataractous mice will attempt to "rescue" the abnormal lens defects. Blastocyst injection of cataractous cells will analyze the contribution of these cells to normal tissue. In vitro studies on clonal populations of mouse lens epithelial cells derived from Cat-Fr, Lop and normal lenses, will characterize morphological changes with replication and growth patterns, e.g. nuclear and cell size, distribution of microtubules, intermediate filaments and microfilaments, synthesis of specific lens proteins. Those in vitro parameters which are found to be associated with certan phases of the normal lens cells replication and/or growth pattern, will serve as a base line for similar studies on the lens epithelial cells derived from the cataractous mice. Somatic cell hybridization and micromanipulation techniques will be employed to study the possibility of manipulating the in vitro parameters.

先天性白内障既是一个眼科问题，也是一个社会问题。这个角色