CHROMOSOME STRUCTURE

拟议的研究旨在理解以下原则 染色体结构。三个具体的问题最初将是 被问到： (I)需要哪些遗传决定或信息 果蝇多线染色体的形成？ (Ii)果蝇染色体11A区的序列是什么 负责“中间异染色质”的性质和 减数分裂重组？ (Iii)人类染色体中的脆性X突变，以及 随后的染色体“印记”导致DNA在 XQ27？ 这项计划与健康的关系如下：问题(一) 可能导致在培养的人类细胞中诱导多丝分裂的方法， 这将为人类基因图谱提供有用的材料； 问题(2)可能提供对减数分裂特性的洞察 适用于人类脆性X的果蝇染色体 染色体；问题(Iii)预计将提供直接 关于人类脆性X突变和 推测的染色体印记事件是导致 人类遗传性智力低下的最常见原因。 方法学涉及细胞遗传学、遗传学和分子分析。 染色体和DNA。重组DNA技术 果蝇的测序、P-元件转化和遗传 本研究将采用分析的方法。

The proposed research is directed at understanding principles of chromosome structure. Three specific questions will initially be asked: (i) What genetic decisions or information are required for formation of polytene chromosomes of Drosophila? (ii) What sequences at Drosophila chromosome region 11A are responsible for the properties of "intercalary heterochromatin" and meiotic recombination? (iii) Does the fragile-X mutation in human chromosomes, and a subsequent chromosome "imprint," lead to late-replicating DNA at Xq27? The health relatedness of this project is as follows: question (i) may lead to methods to induce polyteny in cultured human cells, which would provide useful material for human gene mapping; question (2) may provide insight in meiotic properties of Drosophila chromosomes that are applicable to the human fragile X chromosome; question (iii) is expected to provide direct information about the human fragile X mutation and putative chromosome imprinting event that are responsible for the most common cause of inherited mental retardation in humans. Methodologies involve cytogenetic, genetic, and molecular analysis of chromosomes and DNA. Recombinant DNA technology, DNA sequencing, P-element transformation of Drosophila, and genetic analysis will be used for this research.