CHROMOSOME STRUCTURE
CHROMOSOME STRUCTURE
批准号:
3269545
负责人:
CHARLES D LAIRD
金额:
$19.03万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1980
资助国家:
美国
项目状态:
已结题
起止时间:
1980-03-01 至 1993-11-30
关键词:
DNA Drosophilidae RNA biosynthesis autoradiography biochemical evolution biological polymorphism cell transformation chromatin chromosome aberrations chromosomes cytogenetics electron microscopy fragile X syndromes gene interaction genetic manipulation genetic recombination genetic transcription germ cells heterochromatin human tissue molecular cloning molecular genetics natural gene amplification nucleic acid hybridization nucleic acid sequence regulatory gene structural genes tissue /cell culture transposon /insertion element ultracentrifugation
中文摘要
拟议的研究旨在了解
染色体结构 首先将提出三个具体问题
问道:
(一) 需要哪些基因决定或信息
果蝇多线染色体的形成?
(ii)果蝇染色体11 A区的序列是什么
负责“中间异染色质”的性质,
减数分裂重组?
(iii)人类染色体中的脆性X突变,
随后染色体“印记”,
XQ27?
该项目的健康相关性如下:问题(i)
可能导致在培养的人类细胞中诱导多线性的方法,
这将为人类基因定位提供有用的材料;
问题(2)可以提供对减数分裂特性的了解,
果蝇染色体适用于人类脆性X染色体
染色体;问题(iii)预计将提供直接
关于人类脆性X突变的信息,
假定的染色体印记事件,负责
人类遗传性智力迟钝的最常见原因。
方法学涉及细胞遗传学、遗传学和分子分析
染色体和DNA。 重组DNA技术
测序、果蝇的P-元件转化和遗传
分析将用于这项研究。
英文摘要
The proposed research is directed at understanding principles of
chromosome structure. Three specific questions will initially be
asked:
(i) What genetic decisions or information are required for
formation of polytene chromosomes of Drosophila?
(ii) What sequences at Drosophila chromosome region 11A are
responsible for the properties of "intercalary heterochromatin" and
meiotic recombination?
(iii) Does the fragile-X mutation in human chromosomes, and a
subsequent chromosome "imprint," lead to late-replicating DNA at
Xq27?
The health relatedness of this project is as follows: question (i)
may lead to methods to induce polyteny in cultured human cells,
which would provide useful material for human gene mapping;
question (2) may provide insight in meiotic properties of
Drosophila chromosomes that are applicable to the human fragile X
chromosome; question (iii) is expected to provide direct
information about the human fragile X mutation and
putative chromosome imprinting event that are responsible for the
most common cause of inherited mental retardation in humans.
Methodologies involve cytogenetic, genetic, and molecular analysis
of chromosomes and DNA. Recombinant DNA technology, DNA
sequencing, P-element transformation of Drosophila, and genetic
analysis will be used for this research.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Origins of Variarion in Abnormal FMR1 Methylation in Fragile X Syndrome
-
批准号:7942231
-
项目类别:
-
资助金额:$7.72万
-
财政年份:2009
-
负责人:CHARLES D LAIRD
-
依托单位:
Administrative Core
-
批准号:7942229
-
项目类别:
-
资助金额:$4.59万
-
财政年份:2009
-
负责人:CHARLES D LAIRD
-
依托单位:
Administrative Core
-
批准号:7707264
-
项目类别:
-
资助金额:$15.28万
-
财政年份:2008
-
负责人:CHARLES D LAIRD
-
依托单位:
Origins of Variarion in Abnormal FMR1 Methylation in Fragile X Syndrome
-
批准号:7707252
-
项目类别:
-
资助金额:$15.28万
-
财政年份:2008
-
负责人:CHARLES D LAIRD
-
依托单位:
Shotgun Hairpin-Bisulfite PCR Reveals Genome Methylation and Sequence Variation
-
批准号:7290307
-
项目类别:
-
资助金额:$25.75万
-
财政年份:2006
-
负责人:CHARLES D LAIRD
-
依托单位:
Shotgun Hairpin-Bisulfite PCR Reveals Genome Methylation and Sequence Variation
-
批准号:7491490
-
项目类别:
-
资助金额:$25.75万
-
财政年份:2006
-
负责人:CHARLES D LAIRD
-
依托单位:
Shotgun Hairpin-Bisulfite PCR Reveals Genome Methylation and Sequence Variation
-
批准号:7201948
-
项目类别:
-
资助金额:$25.81万
-
财政年份:2006
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:2193215
-
项目类别:
-
资助金额:$24.7万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:2392269
-
项目类别:
-
资助金额:$26.33万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:2904659
-
项目类别:
-
资助金额:$30.15万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:6181056
-
项目类别:
-
资助金额:$30.09万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:2193216
-
项目类别:
-
资助金额:$25.62万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:6386245
-
项目类别:
-
资助金额:$30.58万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
MOLECULAR GENETICS AND EPIGENETICS OF FRAGILE X SYNDROME
-
批准号:6525769
-
项目类别:
-
资助金额:$31.09万
-
财政年份:1995
-
负责人:CHARLES D LAIRD
-
依托单位:
CHROMOSOME STRUCTURE
-
批准号:3269539
-
项目类别:
-
资助金额:$19.27万
-
财政年份:1980
-
负责人:CHARLES D LAIRD
-
依托单位:
CHROMOSOME STRUCTURE
-
批准号:3269547
-
项目类别:
-
资助金额:$21.89万
-
财政年份:1980
-
负责人:CHARLES D LAIRD
-
依托单位:
CHROMOSOME STRUCTURE
-
批准号:3269543
-
项目类别:
-
资助金额:$15.26万
-
财政年份:1980
-
负责人:CHARLES D LAIRD
-
依托单位:
CHROMOSOME STRUCTURE
-
批准号:3269544
-
项目类别:
-
资助金额:$17.38万
-
财政年份:1980
-
负责人:CHARLES D LAIRD
-
依托单位:
CHROMOSOME STRUCTURE
-
批准号:3269546
-
项目类别:
-
资助金额:$20.22万
-
财政年份:1980
-
负责人:CHARLES D LAIRD
-
依托单位:
CHROMOSOME STRUCTURE AND FUNCTION
-
批准号:3269538
-
项目类别:
-
资助金额:$17.07万
-
财政年份:1980
-
负责人:CHARLES D LAIRD
-
依托单位:
海外基金