POLYSOMES & SUBUNITS: STRUCTURE-FUNCTION RELATIONSHIPS
POLYSOMES & SUBUNITS: STRUCTURE-FUNCTION RELATIONSHIPS
批准号:
3269756
负责人:
ALBERT E DAHLBERG
金额:
$34.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1975
资助国家:
美国
项目状态:
已结题
起止时间:
1975-09-01 至 1993-08-31
关键词:
Bacillus stearothermophilus Escherichia coli R factors antibiotics bacterial RNA bacterial genetics chemical binding chemical structure function density gradient ultracentrifugation drug resistance gel electrophoresis genetic enhancer element genetic manipulation genetic promoter element genetic translation messenger RNA mutant nucleic acid sequence nucleic acid structure polysomes protein biosynthesis ribosomal RNA ribosomal proteins ribosomes streptomycin
中文摘要
本研究计划的目的是确定和
英文摘要
The objective of this research proposal is to identify and
characterize functional sites in E. coli rRNA which are involved
in the process of protein synthesis. Using a plasmid-borne rrnB
operon we are producing mutations in the 16S and 23S rRNA genes by
in vitro and in vivo methods. These mutations will be studied by
a variety of molecular and biochemical techniques. Sites of
interest include the Shine-Dalgarno region near the 3' 16S rRNA
that base-pairs to mRNA and may bs involved in frameshifting, a
potential site in 16S rRNA for the recognition of a translational
enhancer in mRNA, regions in 16S and 23S rRNA involved in subunit
association, and sites in 16S rRNA potentially involved in
switching between the active and inactive forms of the 30S subunit.
Additional regions to be studied include putative tRNA binding and
decoding sites as well as potential sites for missense suppression,
regions in 16S and 23S rRNA which confer resistance to antibiotics
such as spectinomycin, streptomycin, the amino-glycosides, and
thiostrepton, and finally regions in 16S rRNA which are involved
in termination of translation. Lethal mutants will bs produced on
plasmids containing repressible promoters PL and T7. The plasmid-
coded rRNAs will be characterized by maxicells as well as by a
variety of functional assays which measure rate and fidelity of
translation in vivo, including nonsense and missense suppression.
Methods will be used to specifically isolate the plasmid-coded
mutant ribosomes for structural and functional studies using silent
mutations in 16S (at 1192) and 23S (at 1067) rRNA that confer
resistance to spectinomycin and thiostrepton, respectively. These
studies should contribute to our understanding of the functional
role of rRNA during protein synthesis by defining the catalytic
activity of particular regions of the RNA at a molecular level.
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会议论文
Polysomes & Subunits: Structure-Function Relationships
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批准号:7903808
-
项目类别:
-
资助金额:$35.24万
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财政年份:2009
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负责人:ALBERT E DAHLBERG
-
依托单位:
EFFECT OF ASTHMACEDAR ON ASTHMATIC ANIMALS
-
批准号:3500571
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项目类别:
-
资助金额:$4.11万
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财政年份:1985
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负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes and Subunits: Structure-Function Relationship
-
批准号:6525557
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项目类别:
-
资助金额:$59.1万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES & SUBUNITS--STRUCTURE-FUNCTION RELATIONSHIPS
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批准号:2173488
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项目类别:
-
资助金额:$41.08万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:8450936
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项目类别:
-
资助金额:$63.43万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:7275362
-
项目类别:
-
资助金额:$64.51万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:8241064
-
项目类别:
-
资助金额:$65.73万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES AND SUBUNITS--STRUCTURE/FUNCTION RELATIONSHIP
-
批准号:6018445
-
项目类别:
-
资助金额:$43.8万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES & SUBUNITES STRUCTURE-FUNCTION RELATIONSHIPS
-
批准号:3269753
-
项目类别:
-
资助金额:$24.1万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes and Subunits: Structure-Function Relationship
-
批准号:6791300
-
项目类别:
-
资助金额:$60.9万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes and Subunits: Structure-Function Relationship
-
批准号:6383784
-
项目类别:
-
资助金额:$53.39万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:7490572
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项目类别:
-
资助金额:$64.5万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:7885765
-
项目类别:
-
资助金额:$69.05万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES & SUBUNITS--STRUCTURE-FUNCTION RELATIONSHIPS
-
批准号:2173487
-
项目类别:
-
资助金额:$39.5万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES AND SUBUNITS--STRUCTURE/FUNCTION RELATIONSHIP
-
批准号:2770878
-
项目类别:
-
资助金额:$42.6万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES & SUBUNITS: STRUCTURE-FUNCTION RELATIONSHIPS
-
批准号:3269757
-
项目类别:
-
资助金额:$35.61万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES & SUBUNITES STRUCTURE-FUNCTION RELATIONSHIPS
-
批准号:3269752
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项目类别:
-
资助金额:$24.22万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
POLYSOMES AND SUBUNITS--STRUCTURE/FUNCTION RELATIONSHIP
-
批准号:6178862
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项目类别:
-
资助金额:$47.68万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:6984200
-
项目类别:
-
资助金额:$69.96万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
Polysomes & Subunits: Structure-Function Relationships
-
批准号:8053815
-
项目类别:
-
资助金额:$65.73万
-
财政年份:1975
-
负责人:ALBERT E DAHLBERG
-
依托单位:
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