HUMAN POPULATION GENETICS

人类群体遗传学

• 批准号: 3270027
• 负责人: LUIGI L CAVALLI-SFORZA
• 金额: $ 36.34万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270027
• 关键词: DNA; Primates; biochemical evolution; biological polymorphism; evolution; gene frequency; genetic disorder; genetic mapping; genetic markers; genetic registry /resource /referral center; genotype; geographic site; human genetic material tag; human migration; human population genetics; human population growth; linkage mapping; mitochondrial DNA; natural selections; polymerase chain reaction; restriction fragment length polymorphism; sociology /anthropology; southern blotting; statistics /biometry

Our major interest is human evolution, taking advantage of the new developments in molecular genetics. To this aim we study polymorphisms of nuclear DNA, and we want to continue and bring to a reasonable conclusion our tests of 100 restriction fragment length polymorphisms (RFLPs on at least 15-20 aboriginal populations of the entire world. With modern automation techniques, the completion of this part of the project will be faster. Using these RFLP data as a basis of comparison, we want to effect the transition to PCRPs (PCR polymorphisms) which are more efficient, and will allow us to examine more populations. For some categories of PCRPs, which are especially attractive from a technical point of view (CA repeats), there are potential difficulties in interpretation and we prefer to adopt a multiple strategy, by analyzing two or three different types of PCR ;markers. We propose to test initially both CA repeats and ASO (allele- specific oligonucleotides detected PCRPs, and possibly others. It is also important to start with polymorphisms selected on multiracial samples to avoid the bias present in all polymorphisms so far studied, that were initially selected on Europeans. We also plan to continue our studies of parallelism of genetic and linguistic evolutionary trees, and other correlations between genetic and cultural phenomena.

我们的主要兴趣是人类的进化，利用新的 分子遗传学的发展。 为此，我们研究了 核DNA，我们想继续并得出合理的结论， 我们对100个限制性片段长度多态性（RFLPs）进行了检测， 全世界至少有15-20个土著居民。 与现代 自动化技术，该项目的这一部分将完成 快 使用这些RFLP数据作为比较的基础，我们希望影响 向更有效的PCRPs（PCR多态性）过渡， 让我们可以研究更多的人群 对于某些类别的PCRP， 从技术角度来看特别有吸引力（CA重复）， 在解释方面存在潜在的困难，我们倾向于采用 多重策略，通过分析两种或三种不同类型的PCR ;标记。 我们建议首先测试CA重复序列和阿索（等位基因- 特异性寡核苷酸检测PCRPs，可能还有其他。也是 重要的是从多种族样本中选择的多态性开始， 避免目前为止研究的所有多态性中存在的偏倚， 最初选择欧洲人。 我们还计划继续研究遗传学和 语言进化树，以及基因和 文化现象。