MODELS FOR CYTOCHROME P-450 HYDROXYLASES

细胞色素 P-450 羟化酶模型

• 批准号: 3563670
• 负责人: JAMES P COLLMAN
• 金额: $ 19.98万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3563670
• 关键词: X ray crystallography; chemical models; chemical structure function; chemical synthesis; cytochrome P450; electrochemistry; electron probe spectrometry; enzyme model; heme; hydroxylation; metalloenzyme; molecular site; nuclear magnetic resonance spectroscopy; oxygenases; polymers; porphyrins

A series of synthetic iron porphyrins are being prepared for the purpose of modelling the oxygen binding hemoproteins--hemoglobin, myoglobin, and cytochrome P-450. The structures of these synthetic models are being determined by X-ray diffraction and their physical properties (Mossbauer, electronic, and MCD spectra, magnetic moments, and vibrational spectra) are being determined for comparison with the natural hemoproteins. Special emphasis is being given to studies of models for the hydroxylase cytochrome P-450. Synthetic models for the low-spin and high-spin ferric and the ferrous carbonyl stages of P-450 have been characterized. Preparation and structural characterization of synthetic analogues of the deoxy ferrous form are progressing. Ruthenium analogues of these iron porphyrins are being prepared, including a dioxygen complex. A new "capped" porphyrin and an extensive series of binary "face-to-face" porphyrins have been prepared.

为目的准备了一系列合成铁卟啉 建模氧结合血红蛋白 - 血红蛋白，肌球蛋白和细胞色素 P-450。 这些合成模型的结构是由X射线确定的 衍射及其物理特性（Mossbauer，电子和MCD 正在确定光谱，磁矩和振动光谱） 与天然血红蛋白进行比较。 特别强调 羟化酶细胞色素P-450的模型研究。 合成模型 P-450的低旋转和高旋转铁和亚铁羰基阶段 被描述了。 合成的制备和结构表征 脱氧亚铁形式的类似物正在发展。 ruthenium类似物 这些铁卟啉正在制备，包括二氧化物复合物。 一个新 “盖帽”卟啉和一系列二进制的“面对面”卟啉 已经准备好了。