MODELS FOR CYTOCHROME P-450 HYDROXYLASES

细胞色素 P-450 羟化酶模型

• 批准号: 3563670
• 负责人: JAMES P COLLMAN
• 金额: $ 19.98万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3563670
• 关键词: X ray crystallography; chemical models; chemical structure function; chemical synthesis; cytochrome P450; electrochemistry; electron probe spectrometry; enzyme model; heme; hydroxylation; metalloenzyme; molecular site; nuclear magnetic resonance spectroscopy; oxygenases; polymers; porphyrins

A series of synthetic iron porphyrins are being prepared for the purpose of modelling the oxygen binding hemoproteins--hemoglobin, myoglobin, and cytochrome P-450. The structures of these synthetic models are being determined by X-ray diffraction and their physical properties (Mossbauer, electronic, and MCD spectra, magnetic moments, and vibrational spectra) are being determined for comparison with the natural hemoproteins. Special emphasis is being given to studies of models for the hydroxylase cytochrome P-450. Synthetic models for the low-spin and high-spin ferric and the ferrous carbonyl stages of P-450 have been characterized. Preparation and structural characterization of synthetic analogues of the deoxy ferrous form are progressing. Ruthenium analogues of these iron porphyrins are being prepared, including a dioxygen complex. A new "capped" porphyrin and an extensive series of binary "face-to-face" porphyrins have been prepared.

一系列合成的铁卟啉正在制备中，目的是 氧结合血红蛋白的模拟--血红蛋白、肌红蛋白和细胞色素 P-450。这些合成模型的结构是由X射线确定的 衍射及其物理性质(穆斯堡尔谱、电子谱和MCD谱 光谱、磁矩和振动光谱)正在被确定 与天然的血红素蛋白进行比较。特别强调的是 羟基酶细胞色素P-450模型的研究。综合模型 P-450的低自旋和高自旋的铁和亚铁的羰基阶段 被刻画出来了。合成物的制备及结构表征 脱氧亚铁形式的类似物正在取得进展。Ru的类似物 这些铁卟啉正在制备中，包括一种氧气络合物。一种新的 “封端”的卟啉和一系列广泛的二元“面对面”的卟啉 已经做好了准备。