SPATIAL ARRANGEMENT OF FUNCTIONAL SITES IN RIBOSOMES

核糖体延伸率和伸长率的三维分布 启动因子，大亚基和小亚基蛋白，以及特异性 RNA的区域，将被确定。其他功能站点，如 肽基转移酶与新生多肽链的退出位置 也将被映射。亚基将与定向的抗体发生反应 针对特定核糖体蛋白、针对因子或针对特定核糖体蛋白 并用电子显微镜观察。三维 将通过比较子单元的图像(带有附件)来绘制站点地图 抗体)在不同的方向。我们的目标将是将 蛋白质中糖体的三维结构对其功能的影响 综合。小亚基负责识别 启动因子和转录因子参与的信使核糖核酸起始部位 FMET-tRNA，用于结合氨基酰rRNA，用于与大分子结合 亚单位，并用于调节信使的翻译保真度 阅读。大亚基结合氨基酰tRNA的受体茎 进入A位；催化多肽转移，并参与 伸长和易位。通过将三维空间 核糖体蛋白、核糖体因子和核糖核酸区域的分布 已知的生化信息，我们将尝试阐明其结构 蛋白质合成过程中发生的分子事件的各个方面。 还将进行核糖体结构的比较研究，以便 描述原核生物、真核生物和 细胞器核糖体在蛋白质合成中的共同功能。

The three dimensional distributions on the ribosome of elongation and initiation factors, of large and small subunit proteins, and of specific regions of RNA, will be determined. Other functional sites such as the peptidyl transferase and the exit site of the nascent polypeptide chain will also be mapped. Subunits will be reacted with antibodies directed against specific ribosomal proteins, against factors or against specific regions of RNA and observed by electron microscopy. Three dimensional sites will be mapped by comparing images of subunits (with attached antibodies) in different orientations. Our objective will be to relate the three dimensional structure of the tibosome to its function during protein synthesis. The small subunit is responsible for recongnizing the initiation site of mRNA with the participation of initiation factors and fmet-tRNA, for binding aminoacyl rRNAs, for associating with the large subunit, and for regulating the translational fidelity of messenger reading. The large subunit binds the acceptor stem of aminoacyl tRNA's entering the A site; catalyzes peptidyl transfer,and participates in elongation and translocation. By relating the three dimensional distributions of ribosomal proteins, factors and regions of RNA's with known biochemical information, we will attempt to elucidate the structural aspects of the molecular events occurring during protein synthesis. Comparative studies of ribosome structure will also be pursued in order to relate common structural features of prokaryotic, eukaryotic, and organellar ribosomes to common functions in protein synthesis.