CONTROL OF GENE ACTIVITY AND ORGANELLE BIOGENESIS

这个项目的长期目标是确定一个信号如何起源于 细胞外围触发一系列事件，最终导致合成， 细胞内细胞器的组装。 具体来说，我们正在研究 纤毛原生动物四膜虫的分离机制 嗜热菌启动特定基因的有序表达， 纤毛的改造。 已经开发了改进的方法来获得可行的 诱导四膜虫快速（约10倍） 诱导蛋白质合成和特异性相对（约10倍） α和β微管蛋白的协同诱导。 平移和 这种蛋白质一般诱导的转录机制 正在研究微管蛋白的合成和特异性诱导。 多聚腺苷酸 离子RNA分离自脱细胞后80- 100分钟的再生细胞（此时 微管蛋白约占总蛋白质合成的10%）被 用作制备微管蛋白基因序列的cDNA来源 通过重组DNA技术。 然后将利用这些克隆的DNA序列 研究微管蛋白基因的组织及其在细胞周期中的表达， 再生

The long-term goal of this project is to determine how a signal originating at the cell periphery triggers a series of events culminating in the synthesis and assembly of an intracellular organelle. Specifically, we are studying the mechanisms by which deciliation of the ciliated protozoan Tetrahymena thermophila initiates the orderly expression of specific genes required for reformation of cilia. Improved methods have been developed for obtaining viable deciliation of Tetrahymena which induces a rapid (approximately l0-fold) induction of protein synthesis and a specific, relative (approximately l0-fold) co-ordinate induction of alpha and beta tubulins. Translational and transcriptional mechanisms underlying this general induction of protein synthesis and specific induction of tubulin are being examined. Polysomal polyA ion RNA isolated from regenerating cells 80-l00 min. after deciliation (at which time tubulin represents approximately l0% of total protein synthesis) is being utilized as a source of cDNA from which tubulin gene sequences will be prepared by recombinant DNA techniques. These cloned DNA sequences will then be utilized to study the organization of tubulin genes and their expression during regeneration.