Novel strategies for efficient selection of lines with synchronised development by using properties of the circadian clock

利用生物钟的特性有效选择具有同步发育的品系的新策略

• 批准号: BB/V006665/1
• 负责人: Daphne Ezer
• 金额: $ 59.96万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV006665%2F1
• 关键词: Novel; strategies; efficient; selection; lines

The circadian clock helps detect changes in day length, an indication of the season. In turn, this controls the timing of flowering and other developmental traits. However, we do not know the extent to which the circadian clock influences how well-synchronised plant development is. Much of our food waste comes from farmers disposing of vegetables and fruits that do not meet consumer-driven food standards (vegetable is too big/small; fruit is too ripe/not ripe enough, etc). If development was more synchronised, a higher proportion of plants would meet these standards at harvesttime, leading to improved yields. Synchronised flowering is also important for improving the efficiency of pollination.In this proposal, we will investigate how the circadian clock coordinates the response to changes in day length, and how this influences the synchronicity of flowering time. Additionally, we will pilot two strategies for utilising the associations between the circadian clock and synchronised development to improve the efficiency of plant selection (such as during breeding). First, we will build a statistical model to predict flowering time synchronicity, based on the gene expression of a marker gene for the circadian clock in seedlings. This will be used to pre-filter lines of plants, so that only the most promising lines will need to be grown to adulthood. Secondly, we will identify genetic loci that are associated with properties of the circadian clock, response to photoperiod transition, and developmental synchrony. Genetic loci involved in all three processes will be good genetic markers for selection of synchronous lines of plants, because we will have an idea why these genetic loci might be associated with developmental synchrony.Finally, we will develop new Arabidopsis lines that display synchronised flowering, which will be a valuable resource for future research on the genetic origins of synchronisation of developmental processes.

生物钟有助于检测昼夜长度的变化，这是季节的指示。反过来，这又控制着开花的时间和其他发育特征。然而，我们不知道生物钟在多大程度上影响了植物发育的同步程度。我们的大部分食物浪费来自农民处理不符合消费者食品标准的蔬菜和水果（蔬菜太大/太小；水果太熟/不够熟等）。如果发展更加同步，更高比例的植物将在收获季节达到这些标准，从而提高产量。同步开花对提高授粉效率也很重要。在本文中，我们将研究生物钟如何协调对白昼长度变化的反应，以及这如何影响开花时间的同步性。此外，我们将试行两种策略，利用生物钟和同步发育之间的关联来提高植物选择的效率（例如在育种期间）。首先，我们将基于幼苗生物钟标记基因的基因表达，建立一个预测开花时间同行性的统计模型。这将用于预先筛选植物品系，这样只有最有前途的品系才需要生长到成年。其次，我们将确定与生物钟特性、对光周期转换的反应和发育同步性相关的遗传位点。参与这三个过程的遗传位点将成为植物同步性选择的良好遗传标记，因为我们将了解为什么这些遗传位点可能与发育同步性有关。最后，我们将开发新的拟南芥株系，显示同步开花，这将为未来研究发育过程同步的遗传起源提供宝贵的资源。

项目成果

Multiple metals influence distinct properties of the Arabidopsis circadian clock.

• DOI: 10.1371/journal.pone.0258374
• 发表时间: 2022
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Hargreaves JK;Oakenfull RJ;Davis AM;Pullen F;Knight MI;Pitchford JW;Davis SJ
• 通讯作者: Davis SJ

A non-canonical function of Arabidopsis ERECTA proteins and a role of the SWI3B subunit of the SWI/SNF chromatin remodeling complex in gibberellin signaling.

拟南芥 ERECTA 蛋白的非典型功能以及 SWI/SNF 染色质重塑复合物的 SWI3B 亚基在赤霉素信号传导中的作用。

• DOI: 10.1111/tpj.16261
• 发表时间: 2023
• 期刊: for cell and molecular biology
• 作者: Sarnowska E

Cellular localization of Arabidopsis EARLY FLOWERING3 is responsive to light quality.

• DOI: 10.1093/plphys/kiac072
• 发表时间: 2022-09-28
• 期刊: Plant physiology
• 影响因子: 7.4