GENETICS OF MICROTUBULE ASSEMBLY AND FUNCTION

细胞器的运动，e。G.染色体、囊泡等。 对生物学和医学都至关重要。 驱动蛋白是一种 一组机械化学蛋白质，已知可使细胞器沿着移动 微管 直到最近，只有一种原型驱动蛋白 是已知的。 对A. nidulans，S. cerevisiae和D.在过去的一年里， 将驱动蛋白确立为具有类型特异性的蛋白质家族， 功能协调发展的 A. nidulans似乎是 因为它是核分裂所必需的。 这 格兰特建议继续结构和功能 bimC的表征，特别强调了解它如何 在有丝分裂期间起作用。klpA是A. nidulans 也将被定性。 分子遗传学将用于 确定这些结构和功能之间的关系， proteins.

The movement of cellular organelles, e. g. chromosomes, vesicles, etc. is of fundamental importance to biology and medicine. Kinesin is one of a group of mechanochemical proteins known to move organelles along microtubules. Until recently only a single archetypal kinesin protein was known. The description of other kinesin-like proteins in A. nidulans, S. cerevisiae and D. melanogaster during the past year has established the kinesins as a family proteins with type-specific functions. The kinesin-like bimC protein of A. nidulans appears to be of special interest because it is required for nuclear division. This grant proposes to continue the structural and functional characterization of bimC with special emphasis on understanding how it works during mitosis. klpA, a second kinesin-like protein of A. nidulans will also be characterized. Molecular genetics will be used to determine the relationship between structure and function of these proteins.