GENETICS OF MICROTUBULE ASSEMBLY AND FUNCTION

细胞器的运动，如染色体、小泡等。 对生物学和医学具有根本性的重要性。激动素是 一组已知能移动细胞器的机械力化学蛋白质 微管。直到最近，只有一种典型的动蛋白 是众所周知的。对A. 在过去的一年里，尼杜兰、酿酒葡萄球菌和黑腹葡萄球菌已经 将驱动蛋白建立为具有类型特异性的家族蛋白 功能。纽假单胞菌的类Kinesin BIMC蛋白似乎是 具有特殊的意义，因为它是核分裂所必需的。这 格兰特建议继续实施结构性和功能性 BIMC的特征，特别强调了解它是如何 在有丝分裂期间起作用。KlpA，一种节节拟弧菌的第二运动蛋白样蛋白 也将被描述为。分子遗传学将被用于 确定这些分子的结构和功能之间的关系 蛋白质。

The movement of cellular organelles, e. g. chromosomes, vesicles, etc. is of fundamental importance to biology and medicine. Kinesin is one of a group of mechanochemical proteins known to move organelles along microtubules. Until recently only a single archetypal kinesin protein was known. The description of other kinesin-like proteins in A. nidulans, S. cerevisiae and D. melanogaster during the past year has established the kinesins as a family proteins with type-specific functions. The kinesin-like bimC protein of A. nidulans appears to be of special interest because it is required for nuclear division. This grant proposes to continue the structural and functional characterization of bimC with special emphasis on understanding how it works during mitosis. klpA, a second kinesin-like protein of A. nidulans will also be characterized. Molecular genetics will be used to determine the relationship between structure and function of these proteins.