CONTROL OF RIBOSOMAL GENE TRANSCRIPTION

这项研究的长期目标是了解分子 大核糖体RNA转录调控机制的研究 青蛙，非洲爪哇。今后五年的工作重点是 相互作用的反式作用蛋白的纯化和鉴定 与已经确定的DNA序列一起实施这一调控。这些 研究内容包括：1.辅助蛋白的纯化 与增强子和启动子相互作用，克隆它们的基因，以及 确定它们的作用机制。2.结构研究 和RNA聚合酶的调控--3.研究 核糖体RNA转录和生长控制。4.研究其作用机理 核仁的体外组装和拆解。5.将单元格转换为 含有抗药性核糖体基因的人造核仁。6.定位 复制起源(S)及rDNA扩增机理研究。

The long-term goal of this study is to understand the molecular mechanisms that regulate transcription of the large ribosomal RNAs of the frog, Xenopus laevis. In the next five years efforts will be focused on purification and characterization of trans-acting proteins that interact with already identified DNA sequences to effect this regulation. These studies will include: 1. Purification of accessory proteins that interact with the enhancers and promoters, cloning of their genes, and determination of their mechanism of action. 2. Study of the structure and regulation of RNA polymerase I. 3. Study of the relation between ribosomal RNA transcription and growth control. 4. Study the mechanism of nucleolar assembly-disassembly in vitro. 5. Transform cells to make artificial nucleoli with drug resistant ribosomal genes. 6. Locate origin(s) of replication and study mechanism of rDNA amplification.