X-RAY CRYSTALLOGRAPHIC ANALYSES OF PROTEIN STRUCTURES

这项研究将建立精确定义的蛋白质结构系统 通过应用X射线晶体学技术， 为生物化学实验提供了坚实的物理基础。 分析 几种蛋白质的高分辨率将进行，最初将 包括肌酸激酶、相思豆蛋白、过氧化氢酶、铁蛋白、刀豆球蛋白、伴刀豆球蛋白B 和α-淀粉酶。 分离，结晶，一些生化 表征和初步的X射线衍射研究已经 对每一个都进行了。 重点将放在那些蛋白质的最相关的生物利益 （肌酸激酶、相思豆毒素、α-淀粉酶、过氧化氢酶和铁蛋白）与 目的是通过检查它们， 结构在其各自的底物，辅酶，过渡 状态模拟器和效应器，采用差分傅立叶技术。 在 此外，这些研究将研究独特的结构特征，如 糖蛋白、病毒模型和几种独特的四级结构。 本文提出了一种从低分辨率地震记录中提取相位信息的方法。 电子显微照片，以加速X射线结构分析。 此外，还将建立一个系统，用于数字和光学滤波， 电子显微照片。

This research will establish precisely defined protein structural systems through the application of X-ray crystallographic techniques for the purpose of providing a sound physical basis for biochemical experimentation. The analysis of several proteins to high resolution will be undertaken which initially will include creatine kinase, abrin, catalase, ferritin, canavalin, concanavalin B, and alpha-amylase. Isolation, crystallization, some biochemical characterization and preliminary X-ray diffraction investigations have already been conducted on each of these. Emphasis will be placed on those proteins of most relevant biological interest (creatine kinase, abrin, alpha-amylase, catalase and ferritin) with the objective of elucidating their mechanistic features by examining their structures in the presence of their respective substrates, coenzymes, transition state analogues and effectors by employing the difference Fourier technique. In addition, the studies will examine unique structural features such as a glycoprotein, a virus model, and several unique quaternary structures. A method will be developed for extracting low resolution phase information from electron micrographs for the purpose of expediting X-ray structural analyses. In addition, a system will be set up for digital and optical filtering of electron micrographs.