STRUCTURE OF VIRAL RNA AND ITS ROLE IN ASSEMBLY

病毒RNA的结构及其在组装中的作用

• 批准号: 6519951
• 负责人: ALEXANDER MCPHERSON
• 金额: $ 30.2万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6519951
• 关键词: Bromovirus; RNA virus; X ray crystallography; capsid; nucleic acid structure; tobacco mosaic virus; virus RNA; virus assembly

A variety of physical and chemical techniques, chief among them being X-ray crystallography, will be applied to a series of T = 1 and T = 3 icosahedral viruses with the ultimate objectives of (1) deducing the detailed structure of the RNA genomes inside the viruses, (2) identifying important protein - nucleic acid interactions, and (3) devising a model for the assembly and disassembly of the virions consistent with existing biochemical and molecular biological data. The viruses to be studied are satellite tobacco mosaic virus (STMV), satellite panicum mosaic virus (SPMV), turnip yellow mosaic virus (TYMV), Desmodium Yellow Mottle Virus (DYMV), Tomato Aspermy Virus (TAV), Bromegrass Mosaic Virus (BMV), and empty capsids of each of the last four species. These six spherical viruses represent viruses whose structures are best known, for which RNA is visible in the virion, and for which the greatest amount of data and observations exist. Core RNA particles can be prepared by protease digestion of the intact viruses and their unfolding monitored by quasi elastic light scattering. Empty capsids can be made by freeze thawing and used in difference Fourier syntheses to reveal RNA structure. With the integration of chemical and enzymatic probe information and predictions from computer modeling a comprehensive understanding of structure and function will emerge. This research is significant because the conformation of the nucleic acid component of no spherical virus is currently known, and there is presently no comprehensive structural basis for the processes of viral assembly and disassembly.

各种物理和化学技术，其中主要是