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ELECTRON CARRIERS IN CYANIDE-RESISTANT RESPIRATION

抗氰呼吸中的电子载流子

基本信息

批准号：
3273557
负责人：
JAMES N SIEDOW
金额：
$ 7.76万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-01-01 至 1986-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3273557
关键词：
analytical method cellular respiration cyanides electron spin resonance spectroscopy electron transport metalloproteins mitochondria oxygen microelectrode plant physiology stop flow technique succinate dehydrogenase ubiquinone

项目摘要

The purpose of this research is to elucidate the pathway associated with cyanide-resistant oxygen uptake in mitochondria. Cyanide-resistant respiration occurs in a wide range of plants and animals, including pathogenic protozoa of the genus Trypanosoma (sleeping sickness). There is conflicting information in the literature concerning the role of a metal, particularly iron, at the active site of the cyanide-resistant oxidase and the role of ubiquinone in the overall cyanide-resistant pathway. Research will focus on the use of the inhibitor dibromothymoquinone which seems to inhibit electron transfer in mitochondria by reducing the fluidity of the mitochondrial membrane. The effects of dibromothymoquinone on fluidity will be studied using fatty acid spin labels and EPR spectroscopic techniques. Studies of the reduction and oxidation of dibromothymoquinone in plant and animal mitochondria will also be carried out to determine why dibromothymoquinone has such different effects on mitochondria from the two sources. Optical and EPR spectroscopy will be used to study the mechanism of inhibition of the enzyme lipoxygenase by salicylhydroxamic acid and propyl gallate. Similarities exist between lipoxygenase and the cyanide-resistant oxidase so knowledge of how these two compounds inhibit lipoxygenase could give a clearer picture of the cyanide-resistant oxidase.

本研究的目的是阐明与线粒体中抗氰化物的氧摄取。抗氰呼吸发生在广泛的植物和动物中，包括病原性原生动物，锥虫属（昏睡病）。有矛盾的信息，关于金属，特别是铁，在活性物质中的作用的文献，抗氰氧化酶的位点和泛醌在整体中的作用抗氰途径研究将集中在抑制剂的使用二溴百里醌，它似乎抑制线粒体中的电子转移，降低线粒体膜的流动性。的影响将使用脂肪酸自旋标记研究二溴百里醌对流动性的影响， EPR光谱技术。的还原和氧化的研究二溴百里醌在植物和动物线粒体也将进行，确定为什么二溴百里醌对线粒体有如此不同的影响从两个来源。光学和EPR光谱将用于研究水杨基异羟肟酸抑制脂氧合酶的机制，没食子酸丙酯。脂氧合酶和抗氰化物氧化酶，所以了解这两种化合物如何抑制脂氧合酶可以给出抗氰化物氧化酶的更清晰的图像。