F FACTOR-MEDIATED AND OTHER ABORTIVE INFECTIONS

F 因子介导的感染和其他流产感染

• 批准号: 3280662
• 负责人: IAN J MOLINEUX
• 金额: $ 12.38万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280662
• 关键词: Escherichia coli; bacillary dysentery; bacterial genetics; bacteriophage T7; gene expression; genetic mapping; genetic regulation; genetic transcription; genetic translation; mutant; virus infection mechanism

The primary objective of this research is to understand the molecular mechanisms underlying F-mediated restriction of T7 and of mutants of the related organism, bacteriophage T3. Genetic and biochemical studies are being employed to investigate the pleiotropic physiological defects associated with the abortive infection. These include perturbations in gene expression, both transcriptional and translational, an inhibition of phage DNA replication and possible membrane dysfunctions. The work will include studies both on the phage genes and those chromosomal or F-plasmid encoded genes which determine F-mediated restriction. Pseudorevertants of mutant T3 strains that have regained the ability to overcome F-restriction will be analyzed by biochemical means to investigate the intracellular role of the major capsid protein in controlling the transcriptionally-mediated entry of DNA into the cell and in early steps in phage DNA metabolism. These studies will also be conducted in parallel with phage T7, using its abortive infection of Shigella sonnei as a primary biological assay system.

本研究的主要目的是了解分子 F介导的T7和突变体限制的机制 相关生物体，噬菌体T3。 遗传和生化研究是 用于研究多效性生理缺陷 与流产感染有关。 这些包括扰动 基因表达，转录和翻译，抑制 噬菌体 DNA 复制和可能的膜功能障碍。 该工作将 包括对噬菌体基因和染色体或 F 质粒的研究 编码决定F介导的限制的基因。 突变 T3 菌株的假回复体已恢复了 克服F-限制将通过生化手段进行分析以调查 主要衣壳蛋白在控制细胞内的作用 转录介导的 DNA 进入细胞以及早期步骤 噬菌体 DNA 代谢。 这些研究也将同时进行 使用噬菌体 T7，利用其流产的宋内志贺氏菌感染作为主要 生物测定系统。