F FACTOR MEDIATED ABORTIVE INFECTION

F因子介导的流产感染

• 批准号: 3280661
• 负责人: IAN J MOLINEUX
• 金额: $ 8.75万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280661
• 关键词: Escherichia coli; bacteria; bacteriophage T7; female; genetic mapping; genetic regulation; male; mutant; virus genetics; virus infection mechanism

The overall objective of this proposal is to understand the molecular mechanism whereby bacteriophage T7 is restricted upon infection of a male strain of E. coli. This phenomenom is also known as the abortive infection. A new approach is being applied to help solve an old and controversial problem. We have made, via an in vivo recombination experiment, hybrid phages between T7 and the related phage T3. Whereas T7 is restricted by males, T3 is not and these hybrid phages, which are predominately T7 in origin, contain the T3 functions necessary to overcome the abortive infection. We have also recently isolated mutants of T3 which appear to be fully restricted by male strains. Proposed here is a more detailed genetic and physiological characterization of the gybrid phages, designed in particular to separate the two essential functions supplied by T3. These derivatives will be characterized independently on infections of both male and female strains. Similarly the existing T3 mutants, together with others that will be isolated, will be characterized by genetic, physical and physiological criteria. Given these data, in particular knowing precisely which gene products are defective in the T3 mutants, or prevented from functioning (in the case of T7) by the presence of F we can then study the abortive infection itself. For the first time this phenomenom can be investigated by the use of phage mutants and thus the extensive body of knowldege accumulated, on T7 gene products in particular, can be applied to an understanding of the cause of the F factor-mediated abortive infection.

本提案的总体目标是了解分子 噬菌体T7在感染雄性时受到限制的机制 大肠杆菌菌株杆菌 这种现象也被称为流产 感染 一种新的方法正在应用于帮助解决一个旧的， 有争议的问题。 我们通过体内重组 实验中，T7和相关噬菌体T3之间的杂交瘤。 而T7 受雄性的限制，T3不受雄性的限制，而这些杂交后代， 主要是T7的起源，包含T3的功能，必须克服 流产感染。 我们最近还分离出了T3的突变体， 似乎完全受到男性菌株的限制。 这里提出的是一个更 详细的基因和生理特征的杂种， 特别是为了分离两种基本功能， T3 这些衍生物的特征将独立于感染 雄性和雌性都有。 同样，现有的T3突变体， 与其他将被隔离，将以遗传为特征， 身体和生理标准。 根据这些数据，特别是 精确地知道T3突变体中哪些基因产物是缺陷的，或者 由于F的存在而不能发挥功能（在T7的情况下），我们可以 然后研究流产感染本身。 第一次本 可以通过使用噬菌体突变体来研究这种现象， 积累了大量的知识，特别是关于T7基因产物， 可用于理解F因子介导的 流产感染