MECHANISMS OF PHAGE EXCLUSION SYSTEM

本研究的主要目的是了解 T7排氟的分子机制及相关的 噬菌体 这是一个宿主-寄生虫相互作用的模型系统 其中所涉及的基因可以通过遗传方法容易地操纵。 在这个特殊的排斥系统中积累的知识正在被应用于 另一些则是为了确定模型的通用性。 采用经典和分子遗传技术， 研究与以下疾病相关的多效性生理功能障碍： F-排除。 这些包括基因表达的干扰，抑制 DNA复制和细胞膜缺陷。 将确定是否 噬菌体发育的主要阻断是转录介导的 噬菌体DNA进入细胞。 噬菌体蛋白是如何 排斥系统的靶点与E.大肠杆菌DNA促旋酶 研究正常的噬菌体发育。 描述了在这些宿主中改变的突变体的遗传选择 被排除在外的产品。 这些变种人应该 帮助确定噬菌体和质粒编码基因的相互作用位点 并提供关于导致 噬菌体发育停滞。

The primary objective of this research is to understand the molecular mechanism underlying F-exclusion of T7 and related bacteriophages. This is a model system or host-parasite interactions where the genes involved can be easily manipulated by genetic approaches. Knowledge accrued in this particular exclusion system is being applied to others to determine the generality of the model. Classical and molecular genetic techniques are employed to investigate the pleiotropic physiological dysfunctions associated with F-exclusion. These include perturbations in gene expression, inhibition of DNA replication, and membrane defects. It will be determined if the primary block to phage development is the transcriptionally-mediated entry of phage DNA into the cell. How the phage proteins that are the targets of the exclusion system interact with E. coli DNA gyrase in normal phage development is to be investigated. A genetic selection is described for mutants altered in those host products that are involved in the exclusion system. These mutants should help define the site of interaction of the phage- and plasmid-coded genes and provide information on the primary cellular event that causes the arrest in phage development.