Characterization of an infant rabbit model of bacillary dysentery
幼兔细菌性痢疾模型的表征
基本信息
- 批准号:10055925
- 负责人:
- 金额:$ 23.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-07-01 至 2022-06-30
- 项目状态:已结题
- 来源:
- 关键词:ActinsAddressAdultAffectAgeAnimal ModelAnimalsAntibiotic TherapyAntimicrobial ResistanceBacteriaBacterial InfectionsBloodBlood VesselsCaviaCellsCellular biologyCessation of lifeChildColonCytoskeletonCytosolDefecationDevelopmentDiseaseDisease OutcomeDoseDysenteryEndothelial CellsEpithelial CellsExpression ProfilingEyeFecesFibroblastsFoundationsGene ExpressionGenesHemorrhagic colitisHistopathologyHourHumanHuman VolunteersImmuneImmune responseIn Situ HybridizationIn VitroIndividualInfantInfectionInfiltrationIntestinal MucosaIntestinesInvadedKnowledgeLesionLungMapsModelingMolecularMorbidity - disease rateMucous MembraneMusOralOryctolagus cuniculusOutcomeParasitic infectionPathogenesisPathologicPatientsPreventive InterventionPropertyRouteRuptureSeminalShigella InfectionsShigella flexneriSiteSmall IntestinesSymptomsSystemTherapeutic InterventionTissue-Specific Gene ExpressionTissuesTumor-infiltrating immune cellsType III Secretion System PathwayUlcerVirus DiseasesWorkbasecell motilitycell typecombatdesigndiarrheal diseaseenteric pathogenexperienceexperimental studymacrophagemortalitymutantneutrophilnonhuman primatepathogenpathogenic bacteriaresponsetissue culturetooltranscriptome sequencing
项目摘要
Abstract
Dysentery affects hundreds of millions of people in the world each year and is characterized by damage to the
intestinal tissue and rupture of blood vessels, so that visible quantities of blood are lost with defecation.
Dysentery can result from viral, bacterial, or parasitic infections. The intestinal pathogen Shigella flexneri is the
causative agent of bacillary dysentery and is responsible for more than 250 million cases of dysentery
annually, resulting in more than 200,000 deaths. A major challenge in combating bacillary dysentery is the lack
of a small-animal model that recapitulates the symptoms observed in infected individuals. Our group has
recently uncovered that similar to humans, infant rabbits infected with S. flexneri experienced severe immune
cell infiltration, massive ulceration of the colonic mucosa, and bloody diarrhea. In this application, we propose
to explore the infant rabbit model with wild type and mutant S. flexneri through (1) characterization of bacterial
burden and associated histopathology (Aim1) and characterization of host gene expression profiles (Aim 2).
The characterization of the infant rabbit model proposed in this application will provide critical molecular tools
for understanding the mechanisms supporting bacillary dysentery in humans.
摘要
痢疾每年影响世界上数亿人,其特征是损害
肠组织和血管破裂,因此可见数量的血液随着排便而流失。
痢疾可以由病毒、细菌或寄生虫感染引起。肠道病原体福氏志贺菌是
是细菌性痢疾的病原体,造成了2.5亿多例痢疾病例
每年造成20多万人死亡。防治细菌性痢疾的一个主要挑战是缺乏
一个小动物模型,重现了在感染个体中观察到的症状。我们集团
最近发现,与人类相似,感染S.弗氏经历了严重的免疫
细胞浸润、结肠粘膜大面积溃疡和血性腹泻。在本申请中,我们提出
探讨野生型和突变型S.通过(1)细菌的表征
负荷和相关组织病理学(Aim 1)以及宿主基因表达谱表征(Aim 2)。
本申请中提出的幼兔模型的表征将提供关键的分子工具
以了解人类细菌性痢疾的发病机制。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('HERVE F AGAISSE', 18)}}的其他基金
Characterization of virulence factors in shigellosis
志贺氏菌病毒力因子的表征
- 批准号:
10724770 - 财政年份:2023
- 资助金额:
$ 23.75万 - 项目类别:
Characterization of an infant rabbit model of bacillary dysentery
幼兔细菌性痢疾模型的表征
- 批准号:
10203818 - 财政年份:2020
- 资助金额:
$ 23.75万 - 项目类别:
High Throughput Screen for Inhibitors of Shigella Flexneri Dissemination
福氏志贺氏菌传播抑制剂的高通量筛选
- 批准号:
10626880 - 财政年份:2020
- 资助金额:
$ 23.75万 - 项目类别:
High Throughput Screen for Inhibitors of Shigella Flexneri Dissemination
福氏志贺氏菌传播抑制剂的高通量筛选
- 批准号:
10029698 - 财政年份:2020
- 资助金额:
$ 23.75万 - 项目类别:
Characterization of vacuole escape in Shigella infection
志贺氏菌感染中液泡逃逸的特征
- 批准号:
9976031 - 财政年份:2020
- 资助金额:
$ 23.75万 - 项目类别:
Characterization of vacuole escape in Shigella infection
志贺氏菌感染中液泡逃逸的特征
- 批准号:
10088399 - 财政年份:2020
- 资助金额:
$ 23.75万 - 项目类别:
High Throughput Screen for Inhibitors of Shigella Flexneri Dissemination
福氏志贺氏菌传播抑制剂的高通量筛选
- 批准号:
10404510 - 财政年份:2020
- 资助金额:
$ 23.75万 - 项目类别:
Regulation of bacterial pathogen actin-based motility by host cell kinases
宿主细胞激酶对细菌病原体肌动蛋白运动的调节
- 批准号:
8095076 - 财政年份:2011
- 资助金额:
$ 23.75万 - 项目类别:
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