EXPRESSION OF DROSOPHILA A-TUBULIN GENES

果蝇 A-微管蛋白基因的表达

• 批准号: 3279160
• 负责人: PIETER C WENSINK
• 金额: $ 12.17万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3279160
• 关键词: autoradiography; developmental genetics; endonuclease; gene expression; gene mutation; genetic manipulation; genetic mapping; genetic transcription; messenger RNA; nucleic acid sequence; structural genes

This project will investigate how Drosophila controls the expression of its Alpha-tubulin genes. The four genes of this small multigene family offer a good opportunity to investigate the control of tissue- and time-specific transcription and to investigate mRNA characteristics that determine mRNA stability and location. The issues to be dealt with in this investigation are: 1) the location and complexity of DNA sequences that cause one family member's transcription pattern to deiifer from that of another member; and 2) whether or not there are sequences in the mRNAs from different family members that cause them to differ in fate, that is to differ in intercellular transport, in cellular location and in stability. In pursing the first issue, regions and sub-regions will be exchanged between the Alpha-tubulin genes and the resulting genes used to transform Drosophila. The pattern of expression of these altered genes will indicate which sequences are necesssary for a particular tissue- or time- specific pattern of transcription. In pursuing the second issue, portions of the mRNA encoding regions of Alpha-tubulin genes will be excahanged with portions of other genes and then a transformation assay will be used to test the effect of 3 feet and 5 feet untranslated regions, introns and translated regions on the intercellular transport, the location and the stability of Alpha-tubulin mRNAs. The initial subjects for these exchanges will be an Alpha-tubulin gene which codes for a maternal mRNA, one which codes for an mRNA that first appears between egg laying and cellular blastoderm formation and one which appears to be transcribed at all times in all tissues.

这个项目将研究果蝇是如何控制其基因表达的 α-微管蛋白基因。这个小的多基因家族的四个基因提供了一个 研究特定组织和特定时间的控制的好机会 转录并研究决定mRNAs的mRNAs特性 稳定性和位置。这次调查中要处理的问题 是：1)导致一个家族的DNA序列的位置和复杂性 成员的转录模式与另一成员的转录模式不同；以及 2)mRNAs中是否存在来自不同家族的序列 导致他们命运不同的成员，也就是 细胞间运输，在细胞内的位置和稳定。 在寻找第一期的过程中，将交换区域和次区域 在α-微管蛋白基因和用于转化的结果基因之间 果蝇。这些改变的基因的表达模式将表明 哪些序列是特定组织或特定时间所必需的 转录模式。在探讨第二个问题时， α-微管蛋白基因编码区的mRNA将被交换 其他基因的一部分，然后进行转化试验将用于 测试3英尺和5英尺非翻译区、内含子和 细胞间转运的翻译区、位置和 α-微管蛋白mRNAs的稳定性这些交流的初始主题 将是一种α-微管蛋白基因，它编码一种母体信使核糖核酸 在产卵和细胞之间首次出现的信使核糖核酸 胚胚层的形成和似乎一直被转录的胚层 在所有的组织中。