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EXPRESSION OF DROSOPHILA A-TUBULIN GENES

果蝇 A-微管蛋白基因的表达

基本信息

批准号：
3279162
负责人：
PIETER C WENSINK
金额：
$ 14.21万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-07-01 至 1990-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3279162
关键词：
Drosophilidae autoradiography developmental genetics endonuclease gene expression gene mutation genetic manipulation genetic mapping genetic transcription messenger RNA nucleic acid hybridization nucleic acid probes nucleic acid sequence structural genes tubulin

项目摘要

This project will investigate how Drosophila controls the expression of its Alpha-tubulin genes. The four genes of this small multigene family offer a good opportunity to investigate the control of tissue- and time-specific transcription and to investigate mRNA characteristics that determine mRNA stability and location. The issues to be dealt with in this investigation are: 1) the location and complexity of DNA sequences that cause one family member's transcription pattern to deiifer from that of another member; and 2) whether or not there are sequences in the mRNAs from different family members that cause them to differ in fate, that is to differ in intercellular transport, in cellular location and in stability. In pursing the first issue, regions and sub-regions will be exchanged between the Alpha-tubulin genes and the resulting genes used to transform Drosophila. The pattern of expression of these altered genes will indicate which sequences are necesssary for a particular tissue- or time- specific pattern of transcription. In pursuing the second issue, portions of the mRNA encoding regions of Alpha-tubulin genes will be excahanged with portions of other genes and then a transformation assay will be used to test the effect of 3 prime and 5 prime untranslated regions, introns and translated regions on the intercellular transport, the location and the stability of Alpha-tubulin mRNAs. The initial subjects for these exchanges will be an Alpha-tubulin gene which codes for a maternal mRNA, one which codes for an mRNA that first appears between egg laying and cellular blastoderm formation and one which appears to be transcribed at all times in all tissues.

本项目将研究果蝇如何控制其基因的表达， α-微管蛋白基因。这个小的多基因家族的四个基因提供了一个研究组织特异性和时间特异性控制的良好机会转录和研究mRNA的特性，决定mRNA 稳定性和位置。本次调查要处理的问题是：1）导致一个家庭的DNA序列的位置和复杂性一个成员的转录模式与另一个成员的转录模式不同;以及 2)是否存在来自不同家族的mRNA序列他们的命运各不相同，也就是说，细胞间运输、细胞定位和稳定性。在追求第一个问题时，将交换区域和次区域 α-微管蛋白基因和用于转化果蝇这些改变的基因的表达模式将表明对于特定的组织或时间特异性转录模式。在处理第二个问题时， α-微管蛋白基因的mRNA编码区将被挖掘，部分的其他基因，然后转化测定将用于测试3个和5个非翻译区、内含子和翻译区的细胞间运输，位置和 α-微管蛋白mRNA的稳定性。这些交流的最初主题是将是编码母体mRNA的α-微管蛋白基因，编码一种mRNA，它首先出现在产卵和细胞分裂之间，胚盘形成和一个似乎是在任何时候都转录在所有组织中。