SEQUENCE ARRANGEMENT IN EUCARYOTIC DNA AND CHROMOSOMES

真核 DNA 和染色体中的序列排列

• 批准号: 3270610
• 负责人: PIETER C WENSINK
• 金额: $ 20.42万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270610
• 关键词: DNA; DNA binding protein; Drosophilidae; binding proteins; centrifugation; chemical structure function; chromosomes; complementary DNA; developmental genetics; ecdysone; egg proteins; electron microscopy; endonuclease; eukaryote; gel electrophoresis; gene expression; genetic manipulation; genetic mapping; genetic transcription; genetic translation; hormone regulation /control mechanism; laboratory rabbit; messenger RNA; nucleic acid hybridization; nucleic acid sequence; radiotracer; regulatory gene; transcription factor

This investigation is a case study of how a higher organism controls a simple development expression pattern. The genes to be studied are the pair of closely spaced and divergently transcribed yolk protein genes of Drosophila melanogaster. These genes have an expression pattern that is highly differentiated with respect to development of the organism and, though fairly simple, has several specificities: tissue, sex, timing within the lifetime of a cell, and hormonal response. The first objective of the project is to identify components of the mechanism by a combination of: 1) experiments which use in vitro mutagenesis and germ line transformation to identify sequences that are necessary and/or sufficient for aspects of the normal expression pattern; and 2) experiments which use DNA binding assays and cell free- transcription to identify and characterize proteins and DNA sequences which influence the yp expression pattern. The second objective is to examine interactions between identified DNA components. This will be done by manipulating component number, arrangement, and kind and then testing the consequences by germ line transformation, cell free transcription, and physical studies of protein-DNA interactions. The third objective is to determine the developmental specificities of cis-acting DNA elements which control a gene that acts in trans to determine one aspect of the yp1-2 transcription pattern.

这项研究是关于高等生物如何 控制简单的发展表达模式。 基因 所研究的是一对距离很近且发散的 转录的黑腹果蝇卵黄蛋白基因。 这些 基因具有高度分化的表达模式 尊重有机体的发展，虽然相当简单， 有几个特点：组织、性别、一生中的时间安排 细胞和荷尔蒙反应。 该项目的第一个目标 是通过组合来识别机制的组成部分 of: 1) 使用体外诱变和种系的实验 转化以识别必要的序列和/或 足以满足正常表达模式的各个方面；和 2) 使用 DNA 结合测定和无细胞实验 转录以识别和表征蛋白质和 DNA 影响 yp 表达模式的序列。 第二个 目的是检查已识别 DNA 之间的相互作用 成分。 这将通过操纵组件来完成 数量、排列和种类，然后测试结果 通过种系转化、无细胞转录和物理 蛋白质-DNA 相互作用的研究。 第三个目标是 确定顺式作用 DNA 的发育特异性 控制反式作用基因的元件以确定一个 yp1-2 转录模式的一个方面。