SEQUENCE ARRANGEMENT IN EUCARYOTIC DNA AND CHROMOSOMES

这项调查是一个案例，研究了高等有机体如何 控制简单的开发表达式模式。基因决定了 被研究的是一对相距很近而又不同的 果蝇卵黄蛋白基因的转录。这些 基因具有高度分化的表达模式 尊重生物体的发育，虽然相当简单， 有几个特殊性：组织，性别，在生命中的时间 一种细胞和荷尔蒙反应。该项目的第一个目标 是通过组合来确定机构的部件 1)使用体外诱变和胚系的实验 识别必要和/或序列的转换 足以满足正常表达模式的各个方面；以及2) 使用DNA结合分析和细胞外实验- 转录以鉴定和鉴定蛋白质和DNA 影响yp表达模式的序列。第二 目的是检查已鉴定的DNA之间的相互作用 组件。这将通过操作组件来完成 数量、安排和种类，然后测试结果 通过生殖系转化、细胞自由转录和物理 蛋白质-DNA相互作用的研究。第三个目标是 确定顺式作用DNA的发育特异性 控制反式作用的基因的元件 PY1-2转录模式的方面。

This investigation is a case study of how a higher organism controls a simple development expression pattern. The genes to be studied are the pair of closely spaced and divergently transcribed yolk protein genes of Drosophila melanogaster. These genes have an expression pattern that is highly differentiated with respect to development of the organism and, though fairly simple, has several specificities: tissue, sex, timing within the lifetime of a cell, and hormonal response. The first objective of the project is to identify components of the mechanism by a combination of: 1) experiments which use in vitro mutagenesis and germ line transformation to identify sequences that are necessary and/or sufficient for aspects of the normal expression pattern; and 2) experiments which use DNA binding assays and cell free- transcription to identify and characterize proteins and DNA sequences which influence the yp expression pattern. The second objective is to examine interactions between identified DNA components. This will be done by manipulating component number, arrangement, and kind and then testing the consequences by germ line transformation, cell free transcription, and physical studies of protein-DNA interactions. The third objective is to determine the developmental specificities of cis-acting DNA elements which control a gene that acts in trans to determine one aspect of the yp1-2 transcription pattern.