Ion Beam Analysis for the 2020's and Beyond: An Integration of Elemental Mapping and 'omics'

2020 年代及以后的离子束分析：元素测绘和“组学”的整合

• 批准号: EP/R031118/1
• 负责人: Melanie Bailey
• 金额: $ 130.67万
• 依托单位: University of Surrey
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR031118%2F1
• 关键词: Ion; Beam; Analysis; 2020; Beyond

The vision of this Fellowship proposal is to provide a unique capacity internationally, where proteins, metabolites and trace elements can be imaged and co-located at the sub-micron scale, under ambient pressure. This is not available using any other technique and will provide significant benefits to researchers in industry and academia studying the fluxes of metabolites, proteins and other biomarkers in tissues and cells. The Fellowship will develop a new emerging world leader and provide training to a team of researchers in this new field. The vision will be achieved by developing a novel toolbox for molecular speciation, to be used alongside ion beam analysis (IBA). The Fellowship will tackle this challenge with three interconnecting work packages, each investigating a different approach to augmenting the molecular speciation that can be provided alongside or with IBA techniques. These approaches can be summarised as follows: 1. Multimodal mass spectrometry and ion beam trace element imaging; 2. Microscale (point) protein and metabolite characterisation alongside ion beam trace element imaging; 3. Multiplexed ion beam imaging of biomolecules and proteins using antibody-lanthanide tags.This will provide a step change in the UK's capability in the characterisation of biological materials. This proposal will enhance the >£10M investment that EPSRC has recently made in renewing the UK National Ion Beam Centre (UKNIBC) contract and investment in associated equipment (EP/P001440/1; EP/I036516/1), ensure continuing provision of cutting-edge capability in the UK and provide enhanced capabilities at the UKNIBC. It will also ensure the primary benefits go to the needs of UK industry and academia and support the development of applications across RCUK priority areas, with significant economic and societal benefits.

该研究金提案的愿景是在国际上提供一种独特的能力，使蛋白质、代谢物和微量元素能够在环境压力下以亚微米尺度成像和共存。这是使用任何其他技术都无法获得的，将为工业和学术界研究组织和细胞中代谢物，蛋白质和其他生物标志物通量的研究人员提供显着的好处。该研究金将培养一位新的世界领导人，并为这一新领域的研究人员团队提供培训。这一愿景将通过开发一种新的分子物种形成工具箱来实现，该工具箱将与离子束分析（IBA）一起使用。该奖学金将通过三个相互关联的工作包来应对这一挑战，每个工作包都将研究一种不同的方法来增强分子形态，这种方法可以与IBA技术一起提供。这些方法可以总结如下：1.多模式质谱和离子束微量元素成像; 2.微尺度（点）蛋白质和代谢物表征以及离子束微量元素成像; 3.使用抗体-镧系元素标记物对生物分子和蛋白质进行多路离子束成像。这将使联合王国在生物材料特性鉴定方面的能力发生重大变化。该提案将加强EPSRC最近在更新英国国家离子束中心（UKNIBC）合同和相关设备投资（EP/P001440/1; EP/I 036516/1）方面的1000万英镑投资，确保继续在英国提供尖端能力，并在UKNIBC提供增强的能力。它还将确保主要利益满足英国工业和学术界的需求，并支持RCUK优先领域的应用开发，具有显著的经济和社会效益。

项目成果

Exploring New Methods to Study and Moderate Proton Beam Damage for Multimodal Imaging on a Single Tissue Section.

• DOI: 10.1021/jasms.2c00226
• 发表时间: 2022-12-07
• 期刊: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
• 影响因子: 3.2
• 作者: Costa, Catia;de Jesus, Janella;Nikula, Chelsea;Murta, Teresa;Grime, Geoffrey W.;Palitsin, Vladimir;Webb, Roger;Goodwin, Richard J. A.;Bunch, Josephine;Bailey, Melanie Jane
• 通讯作者: Bailey, Melanie Jane

Exploring a route to a selective and sensitive portable system for explosive detection- swab spray ionisation coupled to of high-field assisted waveform ion mobility spectrometry (FAIMS).

• DOI: 10.1016/j.fsisyn.2019.07.009
• 发表时间: 2019-01-01
• 期刊: Forensic science international. Synergy
• 作者: Costa, C;van Es, E M;Bailey, M J
• 通讯作者: Bailey, M J

Distinguishing between Contact and Administration of Heroin from a Single Fingerprint using High Resolution Mass Spectrometry.

使用高分辨率质谱法从单个指纹区分海洛因的接触和施用。

• DOI: 10.1093/jat/bkz088
• 发表时间: 2020
• 期刊: Journal of analytical toxicology
• 影响因子: 2.5
• 作者: Costa C

A Multimodal Desorption Electrospray Ionisation Workflow Enabling Visualisation of Lipids and Biologically Relevant Elements in a Single Tissue Section.

• DOI: 10.3390/metabo13020262
• 发表时间: 2023-02-11
• 期刊: Metabolites
• 影响因子: 4.1

Paper spray screening and liquid chromatography/mass spectrometry confirmation for medication adherence testing: A two-step process.

• DOI: 10.1002/rcm.8553
• 发表时间: 2019-08-14
• 期刊: Rapid communications in mass spectrometry : RCM
• 作者: Costa C;Frampas C;Longman KA;Palitsin V;Ismail M;Sears P;Nilforooshan R;Bailey MJ
• 通讯作者: Bailey MJ