A new platform for multiplexed longterm imaging of cell behaviour during regeneration and development.

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Being able to image cells using microscopy is central to many biological research projects. It is often useful to follow living cells to understand their behaviour. Research tools such as fluorescent proteins are available that let one study the minute details of cells in realtime. However, imaging cells within a dense tissue is particularly difficult as the cells above and below interfere with image capture. To partially overcome this issue microscopes that illuminate samples from the side with a "light sheet" have been developed that reduce interference. Further technology is needed to track cells over long periods such as days. Tissues have special environmental needs in order to stay healthy for long periods, and using too much light on the sample can also be hazardous. In addition, if the cells move we need the microscope to follow them automatically. Our researchers are requesting the purchase of an advanced microscope that overcomes these hurdles to enable successful longterm imaging of dense tissues.