A new platform for multiplexed longterm imaging of cell behaviour during regeneration and development.

再生和发育过程中细胞行为多重长期成像的新平台。

• 批准号: BB/W019450/1
• 负责人: Henry Roehl
• 金额: $ 70.96万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW019450%2F1
• 关键词: new; platform; multiplexed; longterm; imaging

Being able to image cells using microscopy is central to many biological research projects. It is often useful to follow living cells to understand their behaviour. Research tools such as fluorescent proteins are available that let one study the minute details of cells in realtime. However, imaging cells within a dense tissue is particularly difficult as the cells above and below interfere with image capture. To partially overcome this issue microscopes that illuminate samples from the side with a "light sheet" have been developed that reduce interference. Further technology is needed to track cells over long periods such as days. Tissues have special environmental needs in order to stay healthy for long periods, and using too much light on the sample can also be hazardous. In addition, if the cells move we need the microscope to follow them automatically. Our researchers are requesting the purchase of an advanced microscope that overcomes these hurdles to enable successful longterm imaging of dense tissues.

能够使用显微镜成像细胞是许多生物学研究项目的核心。跟踪活细胞以了解它们的行为通常是有用的。诸如荧光蛋白之类的研究工具可以让人们实时研究细胞的微小细节。然而，致密组织内的细胞成像特别困难，因为上面和下面的细胞会干扰图像捕获。为了部分克服这个问题，已经开发出了用“光片”从侧面照亮样品的显微镜，以减少干扰。需要进一步的技术来长时间跟踪细胞，比如几天。为了长时间保持健康，纸巾有特殊的环境需求，在样本上使用过多的光也可能是危险的。此外，如果细胞移动，我们需要显微镜自动跟踪它们。我们的研究人员要求购买一种先进的显微镜，以克服这些障碍，使致密组织的长期成像成功。