Next-Generation Transgenesis in Zebrafish.
斑马鱼的下一代转基因。
基本信息
- 批准号:MR/J001457/1
- 负责人:
- 金额:$ 89.21万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2012
- 资助国家:英国
- 起止时间:2012 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Zebrafish (Danio rerio) is a small tropical fish that has rapidly become an excellent model for biomedical studies. Zebrafish are inexpensive to maintain and breed readily. The young fish are small and relatively transparent which enables researchers to make microscopic observations in the living animal. This research project aims to develop an exciting new technology that will allow us to watch individual cells in the intact animal. To do this we will fluorescently label individual cells which will allow us to follow them using high-resolution microscopy. This new technology will also allow us to target specific molecules within these labelled cells to disrupt their activity. The molecules that we will target play important roles in regeneration, stem cells and cancer. After we disrupt the function of a molecule, we will be able to watch the cell to see whether its movement, division or differentiation potential are affected. For example we can test whether disruption of one molecule in a cell causes it to divide rapidly and form a tumour. This method will even allow us to follow what happens to an individual cell over a period of months or years. These exciting new tools will enable many researchers around the world to answer unresolved questions and will open up many new areas for biomedical research.
斑马鱼(Danio rerio)是一种小型热带鱼,已迅速成为生物医学研究的极好模型。斑马鱼的饲养成本低,繁殖容易。幼鱼很小,相对透明,使研究人员能够在活体动物中进行显微观察。该研究项目旨在开发一种令人兴奋的新技术,使我们能够观察完整动物体内的单个细胞。为此,我们将荧光标记单个细胞,这将使我们能够使用高分辨率显微镜跟踪它们。这项新技术还将使我们能够靶向这些标记细胞内的特定分子,以破坏它们的活性。我们将靶向的分子在再生、干细胞和癌症中发挥重要作用。在我们破坏分子的功能后,我们将能够观察细胞,看看它的运动,分裂或分化潜力是否受到影响。例如,我们可以测试细胞中一个分子的破坏是否会导致它迅速分裂并形成肿瘤。这种方法甚至可以让我们跟踪单个细胞在几个月或几年内发生的事情。这些令人兴奋的新工具将使世界各地的许多研究人员能够回答尚未解决的问题,并将为生物医学研究开辟许多新领域。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Thyroid Hormones Regulate Zebrafish Melanogenesis in a Gender-Specific Manner.
- DOI:10.1371/journal.pone.0166152
- 发表时间:2016
- 期刊:
- 影响因子:3.7
- 作者:Guillot R;Muriach B;Rocha A;Rotllant J;Kelsh RN;Cerdá-Reverter JM
- 通讯作者:Cerdá-Reverter JM
Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration.
- DOI:10.1371/journal.pone.0260372
- 发表时间:2021
- 期刊:
- 影响因子:3.7
- 作者:Marsay KS;Greaves S;Mahabaleshwar H;Ho CM;Roehl H;Monk PN;Carney TJ;Partridge LJ
- 通讯作者:Partridge LJ
Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish.
- DOI:10.1371/journal.pgen.1007260
- 发表时间:2018-04
- 期刊:
- 影响因子:4.5
- 作者:Nagao Y;Takada H;Miyadai M;Adachi T;Seki R;Kamei Y;Hara I;Taniguchi Y;Naruse K;Hibi M;Kelsh RN;Hashimoto H
- 通讯作者:Hashimoto H
Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration
四跨膜蛋白 Cd9b 和 Cxcl12a/Cxcr4b 对集体细胞迁移的控制具有协同作用
- DOI:10.1101/2021.05.19.444012
- 发表时间:2021
- 期刊:
- 影响因子:0
- 作者:Marsay K
- 通讯作者:Marsay K
Conserved and context-dependent roles for pdgfrb signaling during zebrafish vascular mural cell development.
- DOI:10.1016/j.ydbio.2021.06.010
- 发表时间:2021-11
- 期刊:
- 影响因子:2.7
- 作者:Ando K;Shih YH;Ebarasi L;Grosse A;Portman D;Chiba A;Mattonet K;Gerri C;Stainier DYR;Mochizuki N;Fukuhara S;Betsholtz C;Lawson ND
- 通讯作者:Lawson ND
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Henry Roehl其他文献
09-P037 Ret tyrosine kinase signalling is required for the development of a functionally related set of zebrafish head muscles
- DOI:
10.1016/j.mod.2009.06.367 - 发表时间:
2009-08-01 - 期刊:
- 影响因子:
- 作者:
Robert Knight;Katharina Mebus;Henry Roehl - 通讯作者:
Henry Roehl
13-P049 FGF signalling is regulating bone development in zebrafish
- DOI:
10.1016/j.mod.2009.06.522 - 发表时间:
2009-08-01 - 期刊:
- 影响因子:
- 作者:
Katharina Felber;Phil Elks;Peter Croucher;Henry Roehl - 通讯作者:
Henry Roehl
Henry Roehl的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Henry Roehl', 18)}}的其他基金
A new platform for multiplexed longterm imaging of cell behaviour during regeneration and development.
再生和发育过程中细胞行为多重长期成像的新平台。
- 批准号:
BB/W019450/1 - 财政年份:2022
- 资助金额:
$ 89.21万 - 项目类别:
Research Grant
How are regenerative cells recruited during zebrafish larval tail regeneration?
斑马鱼幼虫尾部再生过程中再生细胞是如何招募的?
- 批准号:
BB/S015086/1 - 财政年份:2019
- 资助金额:
$ 89.21万 - 项目类别:
Research Grant
相似国自然基金
Next Generation Majorana Nanowire Hybrids
- 批准号:
- 批准年份:2020
- 资助金额:20 万元
- 项目类别:
相似海外基金
Next Generation Glioma Treatments using Direct Light Therapy
使用直接光疗法的下一代神经胶质瘤治疗
- 批准号:
10092859 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
EU-Funded
Next-generation KYC banking verification via embedded smart keyboard
通过嵌入式智能键盘进行下一代 KYC 银行验证
- 批准号:
10100109 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Collaborative R&D
Multi-component interventions to reducing unhealthy diets and physical inactivity among adolescents and youth in sub-Saharan Africa (Generation H)
采取多方干预措施减少撒哈拉以南非洲青少年的不健康饮食和缺乏身体活动(H 代)
- 批准号:
10106976 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
EU-Funded
Safe and Sustainable by Design framework for the next generation of Chemicals and Materials
下一代化学品和材料的安全和可持续设计框架
- 批准号:
10110559 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
EU-Funded
Next-Generation Distributed Graph Engine for Big Graphs
适用于大图的下一代分布式图引擎
- 批准号:
DP240101322 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Discovery Projects
Next Generation Fluorescent Tools for Measuring Autophagy Dynamics in Cells
用于测量细胞自噬动态的下一代荧光工具
- 批准号:
DP240100465 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Discovery Projects
PhD in the Next Generation of Organic LEDs
下一代有机 LED 博士
- 批准号:
2904651 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Studentship
van der Waals Heterostructures for Next-generation Hot Carrier Photovoltaics
用于下一代热载流子光伏的范德华异质结构
- 批准号:
EP/Y028287/1 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Fellowship
MagTEM2 - the next generation microscope for imaging functional materials
MagTEM2 - 用于功能材料成像的下一代显微镜
- 批准号:
EP/Z531078/1 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Research Grant
FLF Next generation atomistic modelling for medicinal chemistry and biology
FLF 下一代药物化学和生物学原子建模
- 批准号:
MR/Y019601/1 - 财政年份:2024
- 资助金额:
$ 89.21万 - 项目类别:
Fellowship